摘要
利用酶标仪动力学方法测定4个菜区黄曲条跳甲的总酯酶、乙酰胆碱酯酶活性分布频率,并与浸叶法测定的药剂敏感性进行比较分析,结果表明利用酶标动力学方法能够快速测定黄曲条跳甲总酯酶、乙酰胆碱酯酶活性在不同活性区段的频率分布,通过分析总酯酶活性大于3.2D(450nm)/(min·insect)、乙酰胆碱酯酶活性小于0.016D(405nm)/(min·insect)的个体数可以快速监测黄曲条跳甲田间种群的药剂敏感性水平高低。
Frequency distributions of general esterase and acetylcholinesterase in striped flea beetle,phyllotreta striolata,from 4 regions were detected with enzymatic kinetics method in a microplate reader, and were compared with the insecticide susceptibility tested by leaf dipping methods. The results indicated that the former method was a rapid detection of insecticide susceptibility of striped flea beetle by analyzing individual number on the general esterase with an activity of more than 3.2⊿D（450nm）（min·insect）, while acetylcholinesterase less than 0.016⊿D（405nm）（min·insect）.
出处
《中国农学通报》
CSCD
北大核心
2010年第8期226-229,共4页
Chinese Agricultural Science Bulletin
基金
福建省自然科学基金"毒死蜱在黄曲条跳甲灾变中的作用及其机理研究"(B0610025)
关键词
酶标仪动力学方法
黄曲条跳甲
抗性监测
enzymatic kinetics method
striped flea beetle
resistance monitoring
