摘要
在常规的CTAB法基础上,对其中某些步骤和所用药品进行改良,以寻求一种能简便快速地排除多酚类、蒽醌类和甾体类化合物等物质干扰的方法。应用该改良CTAB法所得鹧鸪茶基因组DNA其长度接近21kb,OD260/OD280比值在1.8左右。以此DNA为模板可获得条带清晰、重复性好的ISSR-PCR扩增结果。
Based on the routine CTAB method, some steps and reagents used to obtain DNA, were modified in this study to develop a simple and rapid method for removing the interference of some chemicals, such as polyphenols, anthraquinones, sterids and so on. The molecular weight of DNA, isolated by modified CTAB method, was nearly 21 kb, and the OD260/OD280 was about 1.8. Using the DNA isolated by modified CTAB method as the template in ISSR-PCR, the excellent amplification result, with distinct band and fine repeatability, could be obtained.
出处
《中国农学通报》
CSCD
北大核心
2010年第8期69-73,共5页
Chinese Agricultural Science Bulletin
基金
海南大学校级重点学科专项资金研究课题"海南野生鹧鸪茶资源的种质收集与遗传多样性的分子标记"
关键词
鹧鸪茶
DNA提取
改良CTAB法
多酚
ISSR—PCR
Mallotus oblongiolus （Miq.） Muell.-Arg.
DNA extraction, modified CTAB method
polyphenols：ISSR-PCR
