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Targeted conditional gene knockout in human embryonic stem cells 被引量：6

Targeted conditional gene knockout in human embryonic stem cells
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摘要 We report our application of a promoterless knockout （KO） strategy combined with Flpase- and Cre-mediated recombination to efficiently ablate gene function in hu- man embryonic stem （hES） cells. Using this strategy, we modified and deleted both alleles of BAF250a （ARID 1A）, a signature component of the ATP-dependent SWI/ SNF chromatin remodeling complex BAF. This strategy should facilitate the functional studies of genes essential for hES cell self-renewal and differentiation, and be instrumental for human therapeutic applications.
作者 Lei Bu Xiaolin Gao Xin Jiang Kenneth R Chien Zhong Wang
机构地区 Cardiovascular Research Center
出处 《Cell Research》 SCIE CAS CSCD 2010年第3期379-382,共4页 细胞研究（英文版）
分类号 Q813 [生物学—生物工程] Q78
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参考文献15

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同被引文献66

  • 1卜友泉,杨正梅,宋方洲.新基因功能研究的策略与方法[J].生命科学研究,2006,10(S1):95-98. 被引量：12
  • 2石东乔.“基因敲除”研究进展[J].自然杂志,1996,18(1):33-36. 被引量：3
  • 3张轶文,王少元,林旭,王程毅.家族性急性髓系白血病差异表达基因的筛选与鉴定[J].中华医学杂志,2007,87(8):533-537. 被引量：6
  • 4Kay MA.State-of-the-art gene-based therapies:the road ahead. Nature Reviews Genetics . 2011 被引量：2
  • 5Qiurong Ding,Youn-Kyoung Lee,Esperance A.K. Schaefer,Derek T. Peters,Adrian Veres,Kevin Kim,Nicolas Kuperwasser,Daniel L. Motola,Torsten B. Meissner,William T. Hendriks,Marta Trevisan,Rajat M. Gupta,Annie Moisan,Eric Banks,Max Friesen,Robert T. Schinzel,Fang Xia,Alexander Tang,Yulei Xia,Emmanuel Figueroa,Amy Wann,Tim Ahfeldt,Laurence Daheron,Feng Zhang,Lee L. Rubin,Lee F. Peng,Raymond T. Chung,Kiran Musunuru,Chad A. Cowan.A TALEN Genome-Editing System for Generating Human Stem Cell-Based Disease Models[J]. Cell Stem Cell . 2013 被引量：1
  • 6Nathalie Cartier,Salima Hacein-Bey-Abina,Cynthia C. Bartholomae,Pierre Bougnères,Manfred Schmidt,Christof Von Kalle,Alain Fischer,Marina Cavazzana-Calvo,Patrick Aubourg.Lentiviral Hematopoietic Cell Gene Therapy for X-Linked Adrenoleukodystrophy[J]. Methods in Enzymology . 2012 被引量：1
  • 7Anusara Daenthanasanmak,Gustavo Salguero,Sylvia Borchers,Constanca Figueiredo,Roland Jacobs,Bala Sai Sundarasetty,Andreas Schneider,Axel Schambach,Britta Eiz-Vesper,Rainer Blasczyk,Eva M. Weissinger,Arnold Ganser,Renata Stripecke.Integrase-defective lentiviral vectors encoding cytokines induce differentiation of human dendritic cells and stimulate multivalent immune responses in vitro and in vivo[J]. Vaccine . 2012 (34) 被引量：1
  • 8Frank Soldner,Josée Laganière,Albert W. Cheng,Dirk Hockemeyer,Qing Gao,Raaji Alagappan,Vikram Khurana,Lawrence I. Golbe,Richard H. Myers,Susan Lindquist,Lei Zhang,Dmitry Guschin,Lauren K. Fong,B. Joseph Vu,Xiangdong Meng,Fyodor D. Urnov,Edward J. Rebar,Philip D. Gregory,H. Steve Zhang,Rudolf Jaenisch.Generation of Isogenic Pluripotent Stem Cells Differing Exclusively at Two Early Onset Parkinson Point Mutations[J]. Cell . 2011 (2) 被引量：1
  • 9Mohammed S. Al-Dosari,Xiang Gao.Nonviral Gene Delivery: Principle, Limitations, and Recent Progress[J]. The AAPS Journal . 2009 (4) 被引量：2
  • 10Jizhong Zou,Morgan L. Maeder,Prashant Mali,Shondra M. Pruett-Miller,Stacey Thibodeau-Beganny,Bin-Kuan Chou,Guibin Chen,Zhaohui Ye,In-Hyun Park,George Q. Daley,Matthew H. Porteus,J. Keith Joung,Linzhao Cheng.Gene Targeting of a Disease-Related Gene in Human Induced Pluripotent Stem and Embryonic Stem Cells[J]. Cell Stem Cell . 2009 (1) 被引量：1

引证文献6

二级引证文献12

Cell Research
2010年 第3期

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