摘要
根据乙烯利刺激巴西橡胶树(Hevea brasiliensis)胶乳差异表达cDNA文库的EST序列信息,利用RACE技术从胶乳中成功克隆了1个含有LIM结构域的未知功能新基因HbLIM的全长cDNA。生物信息学分析表明,该基因全长cDNA由1016bp的碱基组成,拥有1个570bp的开放阅读框编码189个氨基酸残基;推测氨基酸序列富含Lys,Ser,Ala和Leu,含有2个LIM结构域和2个N-豆蔻酰化位置。序列相似性分析表明,HbLIM编码的氨基酸序列与蓖麻、杨毛、葡萄相应的LIM基因的相似性高达98%,95%和92%。这些研究结果为深入探讨LIM结构域蛋白在巴西橡胶树乳管发育及乳管代谢中的功能奠定了基础。
Based on the information of EST sequences from latex SSH cDNA library stimulated by ethephon, RACE was used to isolate the full length eDNA of an unknown functional novel gene with two LIM domains from H. brasiliensis, HbLIM. Bioinformatics analysis results showed which comprises of 1 016bp with a 570bp ORF and encodes a 189 animo acid residues. The deduced animo acid sequence with two LIM domains and two Nmyristoylation sites is rich in Lys,Ser,Ala and Leu and has high identity of 98%, 95% and 92% to the homologues from Pollen specific, Populus trichocarpa and Vitis vinifera. These results were very important for elucidation of the （unctions of LIM-domain protein in latercifer development and metabolism in It. brasiliensis.
出处
《热带生物学报》
2010年第1期62-66,共5页
Journal of Tropical Biology
基金
国家自然科学基金(30760917)
中央级公益性科研院所业务费专项资金(ITBBZD0716)
