摘要
对酿酒酵母CGMCC No.2266催化的生物转化反应液中生成的(S)-β-羟基苯丙酸乙酯进行分离纯化。采用溶剂萃取和柱色谱相结合的方法制备(S)-β-羟基苯丙酸乙酯,考察了不同洗脱体系及不同洗脱模式对(S)-β-羟基苯丙酸乙酯柱色谱分离效果的影响。结果表明,转化液经离心弃去微生物细胞后用正己烷萃取上清液,萃取液经蒸馏除去正己烷后获得的样品用硅胶柱进行层析分离,硅胶柱层析的较佳洗脱条件为:洗脱液石油醚与乙酸乙酯的体积比为7:1,洗脱液流速为1.5mL/min,上样量为0.24g,径高比为0.0833。获得的(S)-β-羟基苯丙酸乙酯的纯度为98.2%,对映体过剩值为97.5%。
The high purity （S）-β-hydroxy phenylpropionate（HPPE） was obtained frompreservation of laboratory yeast strains by solvents extraction and silica column chromatography. The effects iof different eluents and eluenting condition on purification of HPPE were also studied. The result showed that the high purity （S）-β- hydroxy phenylpropionate was attained from crude HPPE by silica column chromatography with petroleum etherethyl acetate （7：1,V/V） as eluents, the sample volume was 0.24 g, the ratio of column＇s diameter to height was 0.0833, and the eluant velocity was 1.5 mL/min.
出处
《浙江化工》
CAS
2010年第3期18-21,共4页
Zhejiang Chemical Industry
基金
浙江省科技厅计划项目资助(No.2007C33047)
