摘要
目的应用双向电泳技术和质谱技术研究六味地黄汤对甲亢型肾阴虚大鼠肝线粒体蛋白质组的影响。方法双向电泳分离甲状腺素造成的肾阴虚模型和六味地黄汤治疗大鼠肝线粒体蛋白质，银染后扫描得到蛋白质组图谱，用lmageMaster2D60软件进行差异蛋白质组分析，筛选差异在2倍以上的蛋白质进行质谱鉴定和生物信息学分析。结果大鼠Majorurinary proteinprecursor、Peroxiredoxin-6、内质网蛋A29前体、大鼠转甲状腺素蛋AD链表达升高，78kDa葡萄糖调节篮白前体、谷胱甘肽硫转移酶Mu1、热休克蛋白60、肽基脯氨酰顺反式异构酶、核转录因子-kB2、H＇转运ATP合酶、BNIP1protein表达均降低。结论六味地黄汤滋阴补肾作用机理可能与下调线粒体内H-转运ATP合酶表达量从而减缓能量代谢、抑制NF—kB过量表达和活化以调节炎性细胞因子紊乱状态和凋亡抑制状态、上调PDX6表达对抗氧自由基损伤以及增强性激素分泌水平相关。
Objective Two-dimensional electrophoresis and mass spectrum were used to study the effects of Liuwei Dihuang Decoction on hepatic mitochondria proteome of kidney-yin deficiency rat induced by hyperthyrea. Methods The protein of liver mitochondria from kidney-yin deficiency rats and the kidney-yin deficiency rats treated with Liuwei Dihuang Decoction were isolated by two-dimensional electrophoresis, then stained with silver staining method. The gels were respectively imaged by UMAX ImageScanner and spot-features of proteome difference were analyzed by ImageMaster2D6.0 software. MALDI-TOF MS was used to identify the different protein with abundant changes more than 2 folder between normal rats and kidney-yin deficiency rats. Results In the treated group it was found that major urinary protein precursor, peroxiredoxin-6, endoplasmic reticulum protein 29 precursor, chain D rat transthyretin were increased, while 78 kDa glucose regurated protein precursor, glutathione S-transferase Mul, 60 kDa heat shock protein, peptidyl-prolyl cis-trans isomerase, H -transporting ATP synthase, nuclear factor kappa B 2, BNIP1 protein were decreased. Conclusion The mechanism of nourishing kidney-yin action of Liuwei Dihuang Decoction lies in its relieving energy metabolism, regulating inflammatory factor derangement and apoptosis inhibition, antagonizing free radical damage, enhancing gonadal hormone level by the down regulation of NF-kB, H1-transporting ATP synthase, and the up-regulation of PDX6.
出处
《国际中医中药杂志》
2010年第2期105-107,共3页
International Journal of Traditional Chinese Medicine
关键词
六味地黄汤
线粒体
蛋白质组
NF-KB
Liuwei DihuangDecoction
Mitochondria Proteome Energy metabolism NF-kB
