摘要
目的:探讨尾加压素Ⅱ(urotensinⅡ,U-Ⅱ)对晶状体上皮细胞(lens epithelial cell,LEC)增殖的影响。方法:采用不同浓度的U-Ⅱ与体外培养的LEC共同孵育后,用MTT法检测LEC的活性,并用3H-胸腺嘧啶(3H-TdR)掺入法检测LEC增殖。结果:10-9mol/L U-Ⅱ组、10-8mol/L U-Ⅱ组、10-7mol/L U-Ⅱ组和10-6mol/L U-Ⅱ组的吸光度值均显著高于对照组(P<0.05,P<0.05,P<0.01,P<0.01)。10-10mol/L U-Ⅱ组、10-9mol/L U-Ⅱ组、10-8mol/L U-Ⅱ组、10-7mol/L U-Ⅱ组和10-6mol/L U-Ⅱ组的3H掺入放射活性均显著高于对照组(P<0.01),分别是对照组的1.36倍、1.40倍、1.45倍、1.59倍和1.66倍。呈浓度依赖关系。结论:U-Ⅱ具有促进LEC增殖的作用。
AIM： To study the effect of urotensin Ⅱ（ U-Ⅱ） ,a new biological activity substance, on proliferation of lens epithelial cell（LEC）. METHODS： Different concentrations of U-Ⅱ were incubated with in vitro cultured LEC in vitro. The activities of LEC were detected via methyl thiazolyl tetrazolium （MTT）. The proliferations of LEC were detected via 3H-TdR. RESULTS： The absorbences of groups 10.9 mol/L U-Ⅱ, 10μmol/L U-Ⅱ, 10^-7mol/L U-Ⅱ and 10μmol/L U-Ⅱ were higher than that in control group （P〈 0.05,P〈 0.05, P〈 0.01,P〈 0.01）; the radio-activities of aH-TdR in groups 10^-10μmol/L, 10^-9mol/L U-Ⅱ, 10^-8μmol/L U-Ⅱ, 10^-7 mol/L U-Ⅱ and 10^-6mol/L U-Ⅱ were higher than that in control group （P〈0.01）, which were 1.36, 1.40, 1.45, 1.59 and 1.66 times respectively, with a dose-dependent manner. CONCLUSION ： U- Ⅱ can induce proliferation of LEC.
出处
《国际眼科杂志》
CAS
2010年第3期435-436,共2页
International Eye Science
基金
中国福建省中医药重点资助项目(No.Wzzb0601)~~
