摘要
背景:PI3K是骨骼肌中胰岛素信号转导途径级联反应中关键的蛋白分子之一,其表达异常可影响GLUT4的合成、分泌、移位等变化,从而使血糖升高。目的:通过检测胰岛素和硒联合应用对糖尿病大鼠骨骼肌胰岛素信号转导通路有关蛋白激酶(PI3K、GLUT4)表达的影响。方法:SD雄性大鼠随机分为正常组、糖尿病组、糖尿病+胰岛素组、糖尿病+亚硒酸钠组、糖尿病+胰岛素+亚硒酸钠组。除正常组外,其余4组大鼠腹腔注射链尿佐菌素50mg/kg复制大鼠糖尿病模型。正常组和糖尿病自由进水和进食;糖尿病+胰岛素组按1U/(kg·d)皮下注射胰岛素,糖尿病+亚硒酸钠组按180μg/(kg·d)管饲亚硒酸钠,糖尿病+胰岛素+亚硒酸钠组两药联合用药持续4周;应用免疫印迹和免疫组织化学法检测各实验组骨骼肌胞浆中PI3K和胞膜上GLUT4蛋白表达。结果与结论:免疫组织化学方法所得结果与免疫印迹一致。胰岛素和硒联合应用能明显增加骨骼肌细胞胞浆中PI3K和胞膜上GLUT4蛋白的表达量,说明胰岛素和硒联合应用是通过PI3K途径和增加骨骼肌组织GLUT4蛋白的表达来增强胰岛素信号转导。
BACKGROUND： PI3K is a family of enzymes involved in insulin signal transduction pathway, the abnormal expression of which would affect synthesis, secretion, and migration of GLUT4, therefore, results in increased blood sugar, eventually, leads to diabetes. OBJECTIVE： To discuss the effects of insulin combined with selenium on PI3K and GLUT4 expression in cellular signal transduction of skeletal muscle in diabetic rats. METHODS： Sprague Dawley （SD） rats were randomly divided into the control, diabetic, insulin-treated diabetic, selenium-treated diabetic and combination administration groups. All rats were prepared for diabetic models by injecting 50 mg/kg Streptozocin exception of the control group. Rats in the control and diabetic groups were free to food and water; in the insulin treatment group, rats were subcutaneous injected 1 U/（kg·d） insulin. Rats in the selenium treatment group were treated with a dose of 180 μg/kg per day of sodium selenite dissolved in redistilled water by gavage; and in the combination administration were given both treatments for 4 successive weeks. The levels PI3K and GLUT4 in skeletal muscle were estimated using Western blotting and immunohistochemistry techniques. RESULTS AND CONCLUSION： Immunohistochemistral results were accordance to Western blotting results, which showed the combination of insulin and selenium can remarkable increase the levels of PI3K in skeletal muscle and GLUT4 in skeletal muscle membrane fraction, therefore, enhance the insulin signal transduction pathway.
出处
《中国组织工程研究与临床康复》
CAS
CSCD
北大核心
2010年第7期1213-1217,共5页
Journal of Clinical Rehabilitative Tissue Engineering Research
