摘要
目的分析隐孢子虫在体外不同条件下的脱囊率,以用于隐孢子虫体外培养及免疫学研究。方法以安氏隐孢子虫卵囊为模型,研究比较卵囊保存方法及时间、卵囊脱囊前处理过程、脱囊温度及时间、脱囊液选择等因素对隐孢子虫卵囊脱囊率的影响。结果0.5%次氯酸钠处理可提高卵囊脱囊率,但与未处理组相比差异不显著(P>0.05)。0.75%胆酸盐和0.1%胆汁溶液均可以促进卵囊脱囊,与仅仅使用0.25%胰酶或不使用任何脱囊液组相比差异显著(P<0.05)。卵囊在酸性条件下(pH=3)脱囊率略高于中性条件(pH=7.2),差异不显著(P>0.05),但均显著高于碱性条件(pH=9)(P<0.01)。卵囊脱囊对温度极敏感,24℃水浴卵囊脱囊率极低,显著低于37℃水浴(P<0.01),且37℃条件下卵囊脱囊率随脱囊时间而升高。结论温度、酸碱度、脱囊液是影响隐孢子虫卵囊脱囊率的重要因素,采用先将纯化后的卵囊置于0.5%次氯酸钠溶液4℃作用10min,然后在0.75%胆酸盐溶液中37℃孵育3h,可以得到较高的卵囊脱囊率。
The aim of the present study was to evaluate the effect of storage conditions,pretreatment,temperature,time and excystation solutions on in vitro excystation of Cryptosporidium oocyst.Cryptosporidium andersoni oocyst was used as a model and the results showed that 0.5% sodium hypochlorite could enhance the excystation rates.But there was no significant difference compared with oocysts untreated by sodium hypochlorite（P〉0.05）.0.75% synthetic sodium taurocholate and 1% bile solution could urge the excystation of oocysts,which were significantly different compared with the excystation rate of oocysts in 0.25% trypsin solution or in PBS（〈P0.05）.The excystation rates of oocysts in acidic water（pH =3） were similar with the rates in PBS（pH =7.2） but significantly different from the rates in alkaline water（pH =9）（P〈0.01）.Additionally,the excystation rate of oocysts in water of 24℃ was significantly lower than in water of 37℃（P〈0.01）,and the excystation rate of oocysts raised gradually at 37℃ with the passage of time.It＇s concluded that temperature,acidity and excystation solution were vital factors for the in vitro excystation of Cryptosporidium oocyst.A higher excystation rate could be observed when oocysts were pretreated with 0.5% sodium hypochlorite and treated with 0.75% synthetic sodium taurocholate at 37℃ for 3 hours.
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2010年第3期243-245,251,共4页
Chinese Journal of Zoonoses
基金
国家重大传染病专项-"重要寄生虫病检测和监测技术研究(2008ZX10004-011)"
关键词
隐孢子虫
脱囊
次氯酸钠
脱囊液
Cryptosporidium oocyst excystation sodium hypochlorite excystation solution
