摘要
染色质免疫沉淀(Chromatin immunoprecipitaion,ChIP)技术是分析细胞内生理状态下DNA结合蛋白与基因组DNA相互作用的技术。ChIP与高密度芯片(ChIP-chip)或高通量测序(ChIP-Seq)相结合能产生大量的研究数据,在细胞的基因表达调控网络研究中发挥重要作用。文章主要介绍ChIP、ChIP-chip和ChIP-Seq的技术特点以及发展趋势,重点讨论了ChIP-Seq数据分析方法及相关的应用实例。
Chromatin immunoprecipitation （CHIP） is an effective technique to analyze the interactions of DNA binding proteins with the genome in vivo. ChIP coupled with high density microarray （ChiP-chip） or high-throughput sequencing （ChlP-Seq） has generated large amount of data and expected to allow the development of a network describing the cellular transcriptional regulation. Here, we reviewed the CHIP, ChiP-chip, and ChlP-Seq techniques as well as their perspectives. Focus is given to data analysis of ChlP-Seq and the applications of ChiP-chip and ChlP-Seq.
出处
《遗传》
CAS
CSCD
北大核心
2010年第3期219-228,共10页
Hereditas（Beijing)
基金
国家自然科学基金项目(编号:30871393)资助
