摘要
目的:探讨浙江沿海地区汉族人A型及AB型血型的基因亚型分布情况,为输血及血库血量储存提供理论依据。方法:采用序列特异引物聚合酶链反应(PCR-SSP)检测该地区汉族成人体检者血样本DNA基因亚型,A型280份,AB型110份,根据PCR-SSP反应产生的特异性阳性条带判定相应的基因亚型。结果:A型样本分型成功278份,AB型样本分型成功107份。A型血基因亚型以A/A201、A/O及A201/O较多,占97.84%,而A201/A201及A/A占2.16%;AB型血中基因亚型A/B型(51.40%)及A201/B型(48.60%)所占比例较为接近。A型及AB型血型的基因亚型分布在男女性别之间无显著性差异(P>0.05)。结论:浙江沿海地区汉族人群A型血中存在多种基因亚型,A型血型遗传多态性以及不同亚型之间的抗原性差异需进一步研究。
Objective：To investigate the genotypes of the ABO blood group in the Han nationality of littoral in Zhejiang Province.Methods：Three hundred and ninety blood samples from unrelated Han nationality of littoral in Zhejiang Province including 280 case of blood group A and 110 case of blood group AB,were genotyped by the polymerase chain reaction with sequence-specific primers（PCR-SSP）.Genomics DNA was extracted and amplified by four sequence specific primers,and the typing results were identified corresponding to the special positive strip of PCR reaction.Results：278 blood group A samples and 107 blood group AB samples were genotyped successfully.In group A,genotypes of A/A201,A/O,A201/O accounted for 97.84% of total,while A201/A201 and A/A accounted for 2.16%.In group AB,the frequencies of gene subtype A/B and A201/B were 51.40% and 48.6% respectively.There are no significant difference with blood group A and blood group AB of men and women.Conclusion：The distribution of genotype frequencies of Han nationality of littoral in Zhejiang Province is significantly different with that of other populations.
出处
《中国卫生检验杂志》
CAS
2010年第2期341-342,344,共3页
Chinese Journal of Health Laboratory Technology
关键词
ABO血型
基因分型
序列特异引物聚合酶链反应
ABO blood group
Genotyping
Polymerase china reaction with sequence-specific primers
