摘要
[目的]探讨中医活血、健脾、补肾三法对骨髓间充质干细胞(MSC)移植在结肠炎大鼠肠道定位的影响。[方法]雌性SD大鼠以5%2、4、6-三硝基苯磺酸(TNBs)诱导形成结肠炎模型,造模后随机分为模型对照组、MSC移植组、活血组、健脾组、补肾组,第2天将体外分离培养的雄性SD大鼠MSC经尾静脉注射到各组雌性大鼠体内。疗程结束后取各组大鼠结肠组织,以Y染色体的性别决定区段sry作为标志,以确定MSC的肠道定植情况。[结果]经PCR扩增后,凝胶电泳可以检测到结肠组织雄性性别特异性基因区段sry,MSC移植组阳性率为20%,活血组为35%,健脾组为25%,补肾组为25%。[结论]活血法代表方药桃红四物汤较明显促进MSC在结肠炎大鼠肠道定位。
[Objective]To explore the effect of promoting blood flow,invigorating spleen or kidney recipe on localization of bone marrow mesenchymal stem cell（MSC）in the colon of ulcerative colitis（UC）after transplantation.[Methods]SD female rats were randomly divided into the model group,the MSC transplantation group,promoting blood fiow group,invigorating the spleen group and invigorating the kidney group after the colitis model were induced by Trinitrobenzene sulphonic acid（TNBs）.The MSC from SD male rats were purified by culture expansion in vitro and then were transplanted into SD female rat madels of UC through caudal vein next day.The sex-determining gene（sry） located on Y chromosome was examined by PCR in colon of female rats after the treatment,respectively,to localize the colon MSC.[Results]After using PCR detection,detected colon tissue male gender gene segments sry,transplantion MSC group positive rate was 20%,promoting blood fiow group positive rate was 35%,invigorating the spleen group positive rate was 25%,invigorating the kidney group positive rate was 25%.[Conclusion]Promoting blood flow recipe could promote that MSC localized to the colon of UC rat models.
出处
《中国中西医结合消化杂志》
CAS
2010年第1期15-17,共3页
Chinese Journal of Integrated Traditional and Western Medicine on Digestion
基金
深圳市科技计划资助项目(200802028)
关键词
结肠炎
溃疡性
骨髓间充质干细胞
移植
中医药疗法
ulcerative colitis
bone marrow mesenchymal stem cell
transplantation
traditional chinese medicine therapy
