摘要
采用自行建立的荧光定量PCR对四川省83个鸡场245份临床组织样本中网状内皮增殖症病毒(REV)和禽白血病病毒(ALV)进行了流行病学调查,结果显示REV和ALV的群体阳性率和个体阳性率分别为59.0%、71.0%、86.7%和50.8%,80日龄以下病鸡群REV和ALV的阳性率分别为95.9%和85.9%,REV和ALV的二重感染率为22.9%,表明四川省鸡群中RE和AL普遍存在,是鸡群病因复杂多样,损失严重的重要原因。采用免疫增强剂——黄芪多糖(APS)对部分确诊病例进行治疗和对污染场新引进鸡群进行预防试验,结果表明黄芪多糖对RE和AL有良好的防治效果。
Two hundred and tweanty-five samples from 83 flocks in Sichuan province were detected for molecular epidemiological survey of avian leukosis （AL） and reticuloendotheliosis （RE） with real-time PCR assays developed in our lab formerly. The results showed that flock and individual case positive rates of REV and ALV were 59.0% ,71.0%, 86.7% and 50.8%,respectively. The positive rates of flocks less than 80 days age were 95.9% for REV and 85.9% for LAV. The superinfection rate of REV and ALV was 22.9%. The data indicated that RE and AL were widespread in Sichuau province and intensively involved with complex and diverse diseases and caused heavy losses in commercial chickens. Astragalus polysaccharide （APS） as immunotimulants was used to treat confirmed cases and prevent infection of newborn chicks in comtaminated farms. APS could improve the immunosuppression induced by REV and ALV and had a good curative and preventive effect on these two diseases.
出处
《中国家禽》
北大核心
2010年第6期27-29,共3页
China Poultry
基金
"十一五"国家科技支撑计划项目(2006BAD06A11)
关键词
禽白血病
网状内皮增殖症
流行病学
黄芪多糖
防治
avian leukosis
reticuloendotheliosis
epidemiology
astragalus polysaceharide
treatment
prevention
