摘要
目的了解血平板分离培养细菌L型的特性及其漏检原因。方法201份标本直接接种于血平板培养,盲刮盲扩后取针尖样或云雾状菌落,干片固定观察菌落特征;常规法鉴定L型及其生化特性。结果分离出102株L型(50.7%),针尖样菌落57份,云雾状菌落45份,L型多为颗粒型(G型)菌落(81%),易回复(88%);超薄切片细胞壁缺失;生化反应减弱;金黄色葡萄球菌L型居首位(50%)。结论血平板可直接用于分离培养L型,能保持传统培养L型的多种特性,有利于临床标本同时检出细菌型和L型,可提高L型的检出率。
Objective To investigate the characteristics of bacterial L-forms isolated and cultured with blood plates and the causes of the failure detection.Methods 201 specimens were directly inoculated on blood plates to observe their growth.By blind smearing and blind spreading,the pinpoint-shaped and cloudy colonies were taken and their characteristics were observed through the method of dry-up fixation.Those L-forms were identified with routine methods and their biochemical characteristics were determined.Results 102 strains（50.7%） of L-forms were isolated and identified including 57 pinpoint-shaped colonies and 45 cloudy colonies.They were mainly granular form colonies（G-form,81%）and easy to revert（88%）.The ultrastructures of L-forms were shown to be deficient in cell walls.Biochemical reactions of L-forms decreased.The L-forms of Staphylococcus aureus ranked first（50%）.Conclusions Blood plates are directly used to isolate and culture L-forms.The traditional L-forms characteristics are maintained.It is beneficial to detect the clinical specimens to be bacteria-form and L-forms simultaneously and it can increase the positive rates of detection of L-forms by blood plates.
出处
《检验医学》
CAS
北大核心
2010年第3期185-187,共3页
Laboratory Medicine
关键词
血平板
细菌L型
生物学特性
Blood plate
Bacterial L-form
Biological characteristic
