摘要
对已报道的小麦基因组DNA快速提取方法的部分步骤进行了简化,在水稻上进行了尝试。结果表明,简化法提取的水稻基因组DNA完整性好,PCR扩增效果与试剂盒提取法无明显的差异,结果稳定可靠;而且整个提取过程操作简单、花费时间少,样品用量少,仅需5-10mg,适用于大规模转基因水稻的PCR检测。
The reported method of wheat rapid DNA extraction was simplified at some steps and then applied to rice in this study. The results showed that rice genomic DNA extracted by this simplified method was integral and the PCR amplification using it as tem- plate was stable and reliable,showing no significant difference from plant genomic DNA extraction kit method. Furthermore,the whole extraction procedure is simple,takes less time,and requires a small amount of plant sample about 5 - 10 mg. Therefore this method is suitable for the large-scale PCR detection of transgenic rice.
出处
《生物技术通报》
CAS
CSCD
北大核心
2010年第3期128-130,共3页
Biotechnology Bulletin
基金
国家自然科学基金项目(30671276)
转基因生物新品种培育重大专项(2009ZX08001-032B
2008ZX08001-006)
福建省科技厅重大专项(2008NZ0001-4)
