摘要
目的以新型可注射生物材料壳聚糖β-磷酸三钙为支架，负载骨髓间充质干细胞（MSCs）与富血小板血浆（PRP）构建成新型可注射组织工程骨，观察其体内成骨效应。方法采用中国青山羊双侧胫骨平台下腔穴型骨缺损模型。30只中国青山羊随机分为五组：空白组：骨缺损部不植人任何组织工程材料；单纯材料组：单纯植入组织工程材料壳聚糖β-磷酸三钙；PRP组：植入单纯复合PRP组织工程材料：MSCs组：植人单纯复合MSCs的组织工程材料；PRP／MSCs组：植入复合PRP、MSCs的组织工程材料。于术后第4、8周取出骨缺损区标本进行大体观察和组织学切片观察，图像分析骨缺损区域骨小梁的生成数量。结果术后8周，大体观察显示PRP／MSC组骨缺损区域表面新生骨连续，外观类似正常骨。术后4、8周，组织学显示PRP／MSCs组骨缺损边缘区域类骨质数量明显增多，骨缺损部多为点片状新生骨组织．其中大的片状新生骨组织明显增多。术后4周空白组、单纯材料组、PRP组、MSCs组、PRP／MSCs组的成骨面积百分比分别为8．79±3．63、14．49±3．72、24．18±5．38、24．42±5．10、31．10±3．49：8周时分别为15．41±4．21、25．36±5．37、30．71±4．39、33．97±4．45、48．60±5．97，4周、8周PRP／MSCs组骨修复效果均优于其他各组（P〈0．05）。结论负载PRP和MSCs的新型可注射组织工程骨具有良好的骨修复作用。
Objective To study the effect of a novel injectable scaffold material chitosanbeta-TCP combining bone marrow mesenchymal stem cells （MSCs） and platelet-rich plasma （PRP） on repairing bone defect of goat. Methods The model of the studies was 12mm diameter circular hole tibia bone defect of goat. 30 Chinese goats were raudomly divided into 5 groups： blank group： nothing was embeded in bone defect; simple material group： the material embeded in bone defect was chitosan-beta-TCP; PRP group： the material was chitosan-beta-TCP combining PRP; MSCs group： the material was chitosan-beta-TCP combining MSCs; PRP/MSCs group：the material was chitosan-beta-TCP combining MSCs and PRP. At 4,8 weeks after operation, the samples were observed, histological and image analysis were used to evaluate the effect of bone regeneration. Results At 8 weeks, the surface of bone defect zone of PRP/MSCs group were coverd by continuous new bones, like normal bone. Histological slice showed the osteoid at boundary of normal bone of MSCs/PRP group obviously increased compare to other groups at the 4th or 8th week after operation respectively. The new bone tissues of bone defect were punctiform or lamellar new bone tissues, in which the proportion of big lamellar new bone tissue obviously increased. Image analysis showed that the areas of balnk group, simple material group, PRP group, MSCs group, PRP/MSCs group were 8.79 ± 3.63,14.49 ± 3.72,24.18 ±5.38,24.42 ± 5.10,31.10±3.49 at 4 weeks and 15.41 ± 4.21,25.36 ±5.37,30.71 ± 4.39, 33.97 ± 4.45,48.60 ± 5.97 at 8 weeks respectively. The effect of bone regeneration of PRP/MSCs group was better than other groups （P 〈 0.05）. Conclusion The injectable tissue-engineering bone constructed with chitosan-beta-TCP, MSCs and PRP possesses good ability on repairing bone defect.
出处
《中华显微外科杂志》
CSCD
北大核心
2010年第1期41-45,I0008,共6页
Chinese Journal of Microsurgery
基金
国家高科技研究发展计划（863）重点项目（2003AA205010）
国家自然科学基金（30300367）
广州市科技攻关重大专项子课题（200481-E0031）
关键词
组织工程骨
生物材料
富血小板血浆
骨髓间充质干细胞
Tissue engineering bone
Biocompatible materials
Platelet-rich plasma
Bone marrow mesenehymal stem cells
