摘要
目的探讨两种不同的免疫组织化学方法对实验结果的影响。方法利用二步法和三步法分别在胃癌组织中进行ERα-36染色,比较两种不同的试剂盒在胃癌微阵列芯片上相同组织的表达。结果二步法ERα-36的阳性率为94.59%(105/111),明显高于三步法阳性率为46.85%(52/111)(χ2=4.01,P<0.05);三步法制片结果比二步法背景低,阳性率低,非特异性着色小,结果明确易读;而二步法较三步法操作简单,检查阳性率高,阳性强度高。结论三步法虽然实验耗时比较长,但实验结果较好,二步法较易出结果,假阳性率高。
Objective To investigate the effects of experimental results by two kinds immunohistochemical methods. Methods Using two-step and three-step method were carried out in gastric cancer ERa-36 staining to compare express of cancer microarray in identical tissues by two different kits. Results The positive rate of 94.59% （105/111） in two-step ERa-36 were significantly higher than the three-step 46.85% （52/111）（X^2=4.01, P〈0.05）; The index of background, positive rate, non-specific color in three-step methods were lower than that two-step methods; The index of positive rate, positive strength in two-step methods were higher than that three-step methods. Conclusion Although the experimental time were long, but the experimental results better in three-step, two-step easier to produce results, but the false positive rate were high.
出处
《中国医药指南》
2010年第8期29-30,共2页
Guide of China Medicine
关键词
免疫组织化学技术
二步法
三步法
组织芯片技术
Immunohistochemistry techniques
Two-step method
Three-step method
Tissue microarray technique
