摘要
目的:观察重组质粒Psilencer1.0-U6-si RNA-stat3对移植静脉内膜增生的影响,为预防冠状动脉旁路移植术后移植血管再狭窄提供新的方法。方法:40只雄性Wistar大鼠随机分为空质粒组和重组质粒组,每组20只。脂质体Lipofectamine2000与该空质粒或者重组质粒混合15min再与生物蛋白胶混合后,转染大鼠移植静脉(右颈外静脉中段),在3、7、14和28d时,经左心室灌注4%甲醛并提取移植静脉,采用免疫组织化学方法(增殖细胞核抗原PCNA)检测移植静脉内膜血管平滑肌细胞(VSMCs)的增生及表达,以增殖指数表示。采用HE法检测移植静脉新生内膜增生病理形态学变化,在40倍镜下测量其内膜厚度。结果:免疫组织化学检测,术后7和14d空质粒组移植血管管壁内膜层可见许多PCNA阳性细胞,重组质粒组PCNA阳性细胞数明显少于空质粒组。7d时重组质粒组增殖指数(23.3±2.8)低于空质粒组(31.3±4.7)(P<0.05);14d时重组质粒组增殖指数(15.6±0.4)低于空质粒组(27.2±5.7)(P<0.05)。7d时重组质粒转染组内膜厚度[(0.50±0.02)μm]明显低于空质粒组[(0.90±0.02)μm](P<0.05)。结论:重组质粒Psilencer1.0-U6-si RNA-stat3对冠状动脉旁路移植术后移植静脉内膜增生有明显的抑制作用。
Objective To investigate the influence of the recombinant plasmid Psilencer1.0-U6-siRNA-stat3 on neointima formation of vein graft and develop a new way to prevent restenosis of the vessel graft after arterial bypass.Methods 40 Wistar rats were divided into 2 groups（n=20）：experiment group and control group.The recombinant plasmid Psilencer1.0-U6-siRNA-stat3（exeperiment group） and scramble plasmid（control group） were combined with the liposomes Lipofectimine 2000 and were transfected into vein graft mediated by bioprotain gel via adventitia.On day 3,7,14,28,the vein graft interposed into the arterilized environment were extracted after irrigating 4% formaldehyde through left ventricle in 100 mmHg.The change of neointimal thickening was analyzed by pathological（HE） study and the proliferation of vascular smooth muscle cells（VSMCs） in neointima was detected by immunohistochemistry（proliferating cell nuclear antigen,PCNA）.Results The immunohistochemistry results showed that,on day 7 and 14 after operation,there were more PCNA positive cells in neointima of vein graft in control group than that in experiment group.The PCNA index in experiment group（23.3±2.8） was lower than that in control group（31.3±4.7）（P〈0.05） 7 d after operation.The PCNA index in experiment group（15.6±0.4） was also lower than that in control group（27.2±5.7）（P0.01） 14 d after operation.The morphological result showed that the thickness of neointima experiment group（0.50μm±0.02μm） was thinner than that in control group（0.90μm±0.02μm）（P〈0.05）.Conclusion The recombinant plasmid Psilencer1.0-U6-siRNA-stat3 can inhibit the neointimal formation of vein graft obviously.
出处
《吉林大学学报（医学版）》
CAS
CSCD
北大核心
2010年第2期299-302,I0003,共5页
Journal of Jilin University：Medicine Edition
基金
吉林省科技厅科技发展计划项目资助课题(20070720-2)
关键词
重组质粒
移植静脉
内膜增生
recombinatnt plasmid
vein graft
intinal hyperplasia
