摘要
目的:采用ICR小鼠通过三种行为学实验观察乌灵菌的抗焦虑作用。方法:给药组分别口服乌灵菌粉低、中、高三个剂量0.25、0.50和1.00(g/kg),阳性药对照组为丁螺环酮(2mg/kg)和地西泮(1.5mg/kg),对照组给予等量生理盐水。给药7天后开始行为学实验,包括高架十字迷宫实验、爬梯实验和抗戊四唑致(PTZ)惊厥实验。结果:高架十字迷宫观察5min内小鼠进入开臂的次数(OE),发现乌灵胶囊中剂量组与丁螺环酮组均明显增加(P<0.05,P<0.01),在开臂停留时间(OT)分别占进入两臂总次数和在两臂停留总时间的百分比(OE%和OT%),三剂量组与丁螺环酮组均比对照组明显增加(P<0.01)。记录3min小鼠站立次数和爬梯次数,与对照组比,丁螺环酮组的站立次数和爬梯次数均明显减少(P<0.01),而乌灵胶囊低、高组的站立次数亦明显减少(P<0.05,P<0.01),爬梯次数未见统计学意义。在抗PTZ试验中,中剂量乌灵胶囊明显延长了惊厥发作潜伏期和显著降低了惊厥评分(P<0.01)。结论:三种行为学试验均表明,乌灵胶囊有明确的抗焦虑作用。
Objective： To observe anxiolytic effect of Xylaria nigripes through three behavioral tests in ICR mice. Methods： The mice were orally administered with Xylaria nigripes at different doses of 0. 25, 0. 50 and 1.00 （g/kg） respectively. The mice in positive reference group were treated with Buspirone （2mg/kg） or Diazepam （1. 5mg/kg） and saline was given to control group. 7 days after administration, all the mice were subjected to the behavioral tests, including elevated plus -maze, staircase and PTZ induced convulsion test. Results ： OE were significantly increased in Xylaria nigripes group （ P 〈 0. 05 ） and Buspirone group （ P 〈 0. 01 ） within 5 min , and OE% and OT% were increased remarkably in three Xylaria nigripes treatment group as well as Buspirone group compared to control group （ P 〈 0. 01 ）. Number of rearing and climbing was significantly reduced in Buspirone group compared to control group （ P 〈 0. 01 ）, while the frequency of rearing was remarkably reduced in low and high groups （ P 〈 0. 05, P 〈 0. 01 ）. The frequency of climbing was not significant. In Xylaria nigripes group, the latency of convulsion seizure was remarkably prolonged, and the score of convulsion was lowered in the anti -PTZ test （P 〈 0. 01 ）. Conclusion： Resuhs of behavioral tests showed that Xylaria nigripes exerted a significant anxiolytie effect.
出处
《中国民族民间医药》
2010年第5期27-28,30,共3页
Chinese Journal of Ethnomedicine and Ethnopharmacy
基金
科技部重大新药创制专项资助(编号2009ZX09303)
关键词
乌灵胶囊
抗焦虑
行为学实验
小鼠
Xylaria nigripes
anxiolytic
effect behavioral test
mice
