摘要
研究了α-萘酚与TritonX．100之间的共振能量转移的最佳条件，建立了TritonX-100共振能量转移荧光猝灭法测定α-萘酚的新方法。在pH7．5的Tris-HCl缓冲溶液中，在300．8nm，TritonX-100的荧光猝灭强度与α-萘酚浓度成正比，工作曲线的线性范围为0．36～1．80μg/L，方法的检出限为0．12ng／mL，RSD为0．7％-1．1％，平均加标回收率为100．9％（n=6）。该方法已用于尿样测定，结果满意。
The optimum conditions for the resonance energy transfer between α-naphthol and Triton X-100 were studied, and a novel method for the determination of α-naphthol has been developod based on the resonance energy transfer fluorescence quenching effect of Triton X-100, the fluorescence quenching intensity of Triton X-100 at 300.8 nm was proportional to the concentration of α-naphthol. The linear range of the calibration curve was 0.36 - 1.80 μg/L in the pH 7.5 Tris-HCl buffer solution with the detection limits of 0.12 ng/mL. The relative standard deviation was 0.7% - 1.1% and the average recovery was 100.9%. The proposed method has succeeded in analyzing the spiked urine sample with satisfactory results.
出处
《应用化工》
CAS
CSCD
2010年第3期453-455,共3页
Applied Chemical Industry
基金
湖南省自然科学基金课题(03JJY3030)
湖南省卫生厅科研基金课题(B2003-077)
