摘要
采用常规真菌分离法对健康茶树叶片叶面真菌和内生真菌进行分离,比较了叶面真菌和内生真菌的种群和数量差异。结果表明:从茶树品种黄旦(HD)共分离得到叶面真菌26个形态型,内生真菌18个形态型;从铁观音(TGY)共分离获得叶面真菌16个形态型,内生真菌15个形态型;分离所得真菌大多数为半知菌和子囊菌,少数为担子菌。采用稀释平板法和叶片组织黏附法两种不同的真菌分离方法,获得叶面真菌优势种的种类基本一致,但各种菌的数量差异明显;对黄旦和铁观音两品种比较,采用叶片组织黏附法分离所得叶面真菌的数量没有差异,但采用稀释平板法所获不同真菌的数量差异明显。真菌分离结果表明在茶树叶片表面和内部组织中生长有显著不同的真菌群落。
In the present study, fungal epiphytes and endophytes were isolated from healthy leaves of Camellia sinensis with routine isolation methods. And the phyllosphere fungal communities were evaluated. The results showed that a total of 26 and 18 fungal morphospecies were isolated from the leaf surface and interior of the cultivar Huangdan（HD）, and a total of 16 and 15 fungal morphspecies were isolated from the leaf surface and interior of the cultivar Tieguanyin（TGY）, respectively. The majority of isolated fungi from phyllosphere belonged to Deuteromycetes and Asc0mycetes, with a few belonged to Basidiomycetes, The morphspecies isolated from tea plant leaves were basicly the same with two isolation methods, but the quantity of individual fungus was significantly different. The results also revealed that the species composition of fungi differed greatly between the interior and surface of leaves.
出处
《江西植保》
2010年第1期18-22,24,共6页
Jiangxi Plant Protection
基金
福建省自然科学基金重点项目资助(B0620005)
关键词
茶树
叶面真菌
内生真菌
分离
群落结构
Camellia sinensis, epiphytes
endophytes
isolation
community structure
