摘要
用β-环糊精和对甲苯磺酰氯合成单-(6-O对甲苯磺酰基)β-环糊精,以合成的β-环糊精衍生物为主体,核糖核酸酶为客体,对比研究β-环糊精衍生物催化核糖核酸的水解和β环糊精衍生物非共价修饰的核糖核酸酶催化核糖核酸水解的酶活性,分析其催化活性的高低,并对其作用机理进行初步的探讨.β-环糊精衍生物具有水解核糖核酸的催化活性,能够非共价结合在核糖核酸酶上,形成的非共价修饰核糖核酸酶的催化活性比核糖核酸酶要高8.8倍.
It is a new idea that β-cyclodextrin derivatives can be used to non-covalent modification of the enzymes. In present study, ribonuclease was modified through noncovalent interaction with β-CD-6-OTs, and the enzyme activity improvement was checked. The Mono-（6-O-tosyl）β-CD, （β-CD-6-OTs） synthesized by the reaction between β-Cyclodextrin（β-CD） and p-toluene sulfonyl chloride. The catalysis activity for nucleic acid hydrolysis was determined in four cases： separate β-CD-6-OTs or ribonuclease, and the non-covalent complex of β-CD-6-OTs with normal or denatured ribonulease. Separate β-CD derivatives have a certain catalyzed effect, and its catalysis activity is higher than individual ribonulease. The highest activities was belong to non-covalent complex between β-CD-6-OTs and denatured ribonulease which is higher 8. 8 folds than that of ribonuclease.
出处
《华中师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2010年第1期76-79,共4页
Journal of Central China Normal University：Natural Sciences
基金
中南民族大学植物遗传与发育创新团队资助项目
