摘要
探讨脂联素基因表达缺陷对ApoE基因敲除（ApoE^-/-）小鼠糖脂代谢的影响。注射短发卡RNA（shRNA）腺病毒后高脂喂养的ApoE^-/-小鼠（ADI组）血浆脂联素水平明显低于普食喂养（NF）、单纯高脂喂养（HF）和空载腺病毒高脂对照组（GF，均P〈0．01）。3组高脂喂养的ApoE^-/-小鼠体重、空腹血糖、血浆游离脂肪酸、总胆固醇、甘油三酯、低密度脂蛋白胆固醇（LDL—C）、高密度脂蛋白胆固醇（HDL—C）和血浆胰岛素水平明显高于NF组（均P〈0．01），而ADI组与HF和GF组相比，游离脂肪酸、总胆固醇、甘油三酯、LDL—C、HDL—C和血浆胰岛素水平增高更为明显（P〈0．01或P〈0．05），提示长期高脂饲养和脂联素基因表达缺陷使ApoE^-/-小鼠具备较为典型的糖脂代谢紊乱和胰岛素抵抗的病理生理表现。
The effect of RNA interference-mediated adiponectin gene silence on glucose-lipid metabohsm in apolipoprotein E gene knock-out（ ApoE^-/-）mice was studied. The plasma adiponectin level in ApoE^-/- mice with high-fat diet and adiponectin short hairpin RNA （ shRNA ） adenovirus injection （ ADI group ） was significantly lower than that in ApoE^-/- mice with high-fat diet （HF group）, normal-chow（ NF group）and naked adenovirus injection （ GF, both P〈0.01 ）. Fasting blood glucose, fasting plasma insulin, free fatty acids, total cholesterol, triglycerides, low-density lipoprotein-cholesterol （ LDL-C ）, high-density lipoprotein-cholesterol （ HDL-C ）, and body weight were significantly elevated in three high-fat diet groups compared with in NF group, （P〈0.01）. However,fasting plasma insulin, free fatty acids, total cholesterol, triglycerides, LDL-C, and HDL-C in ADI group were significantly higher than those in HF and GF groups（ P〈0.01 or P〈0.05 ）. A typical clinical phenotype of glucose-lipid metabolism and insulin resistance in ApoE^-/- mice can be induced by high-fat feeding and adiponecting gene silencing.
出处
《中华内分泌代谢杂志》
CAS
CSCD
北大核心
2010年第2期143-145,共3页
Chinese Journal of Endocrinology and Metabolism
基金
基金项目：国家自然科学基金项目（30771037、30370671）
关键词
RNA干扰
脂联素
胰岛素抵抗
糖脂代谢
RNAi
Adiponectin
Insulin resistance
Glucose-lipid metabolism
