摘要
目的研究静脉注射含饱和氢气生理盐水对小鼠肾脏缺血再灌注（IR）损伤的保护作用及其机制。方法健康、雄性的C57BL／6小鼠随机分为3组，每组10只。假手术组（SO组）小鼠仅接受中线开腹、双侧肾蒂游离及关腹操作；缺血再灌注组（IR组）小鼠用无损伤动脉夹同时钳夹双侧肾蒂，阻断45min，制成肾脏IR损伤模型，并于肾脏缺血同时经尾静脉注射生理盐水，5ml／kg；实验组小鼠制成肾脏IR损伤模型，并于肾脏缺血同时经尾静脉注射含饱和氢气生理盐水，5ml／kg。各组小鼠于肾脏再灌注6h时检测血清尿素氮（BUN）和肌酐（Scr）；检测肾组织中丙二醛（MDA）和髓过氧化物酶（MPO）的含量；观察肾脏组织形态学变化并检测肾小管上皮细胞的凋亡情况；观察肾组织中巨噬细胞的浸润情况；检测各组小鼠肾组织中肿瘤坏死因子α（TNF-α）、白细胞介素6（IL-6）、IL-1β和IL-17 mRNA的水平。结果实验组血清BUN和Scr水平明显低于IR组（P〈0．05）。实验组肾组织病理改变较IR组明显减轻，其肾小管损伤评分明显低于IR组（P〈0．01），肾小管上皮细胞凋亡明显轻于IR组（P〈0．05）。实验组肾组织内MDA含量低于IR组（P〈0．05）。实验组小鼠肾组织内中性粒细胞和巨噬细胞的浸润较IR组减少（P〈0．05）。实验组TNpa、IL-6、IL-1β和IL-17 mRNA的水平均低于IR组（P〈0．05）。结论静脉注射含饱和氢气生理盐水能够在一定程度上减轻。肾脏IR损伤，其机制可能与抑制。肾脏IR后炎症反应有关。
Objective To evaluate the protective effect of hydrogen-rich saline on renal ischemia/reperfusion （I/R） in mice. Methods Thirty C57BL/6 mice were randomly divided into 3 groups： sham-operated （SO） group, I/R group （mice were injected with 5 ml/kg saline by tail vein just before ischemia induction） and hydrogen-rich saline group （mice were injected with 5 ml/kg hydrogen-rich saline）. At the 6th h after reperfusion, the sera and renal samples subject to IR injury were collected. The Scr and BUN levels in serum were determined and renal histological changes were also examined. The apoptosis of renal tubular epithelial cells was measured by terminal deoxynucleotidyl transferase mediated dUTP nick end labeling assay. Malondialdehyde （MDA） contents in renal samples were measured using specific kits. The infiltration of F4/80 positive macrophages and neutrophils was assayed by using immunohistochemistry. The mRNA expression of TNF-α, IL-6, IL-1β and IL-17 was detected by using real time reverse transcription PCR. Results As compared with IR group, at the 6th h following reperfusion the levels of Scr and BUN were significantly reduced （P〈0. 05）, histological changes obviously alleviated （P〈0. 01）, apoptosis of renal tubular epithelial cells and MDA contents was decreased （P（0. 05） in hydrogen-rich saline group. Moreover, the infiltration of macrophages and neutrophils, and the mRNA expression of TNF-α, IL-6, IL-1β and IL-17 in renal tissue in hydrogen-rich saline group were also declined as compared with IR group （P〈0. 05）. Conclusion Hydrogen-rich saline can ameliorate renal IR injury to some extent, which is associated with inhibition of inflammatory response induced by reperfusion.
出处
《中华器官移植杂志》
CAS
CSCD
北大核心
2010年第2期109-113,共5页
Chinese Journal of Organ Transplantation
关键词
氢
氯化钠
再灌注损伤
小鼠
肾
Hydrogen
Sodium chloride
Reperfusion injury
Mice
Kidney
