摘要
通过对几种不同的木聚糖提取方法的比较，确立了酸预处理后加水蒸煮的方法提取低聚木糖生产用木聚糖的工艺路线。首先将玉米芯用质量浓度为１ｇ／Ｌ的Ｈ２ＳＯ４在６０℃下浸泡１２ｈ，滤去浸泡液，然后加水至固液比为１∶１０，在１５０℃蒸煮３０ｍｉｎ．采用此工艺木聚糖的提取得率可达到１７％（按玉米芯计），提取液的还原糖与总糖质量之比小于３３％．该法得到的提取液（和渣一起）用自制的木聚糖酶水解，可获得ｍ（木糖）∶ｍ（木二糖）∶ｍ（木三糖）＝１∶５∶２．７的高纯度低聚木糖产品，产品总糖的得率达到２６．４％（按玉米芯计）。
The process for extracting xylan from corncob for the production of xylooligosaccharides was established. This process consisted of pretreatment by acid and steaming. The optimum conditions were as follows presoaked 100 g corncob in 500 g 0.1% H 2SO 4 at 60 ℃ for 12 h,followed by filtration and washing with tap water to about pH6, then steamed at 150 ℃ for 0.5 h (100 g corncob in 1000 mL water). The yield of xylan was about 17% of corncob. Hydrolyzed by specific xylanase to the extract, the cumulative yield of xylooligosaccharides was 26.4% of corncob and the ratio of xylose∶xylobiose∶xylotriose was 1∶5∶2.7.
