摘要
以番茄新品种T431、1911、0701为材料,以9天苗龄的叶片为受体,通过农杆菌Ri质粒介导,将几丁质酶基因和β-1,3葡聚糖酶基因转化番茄。共获得再生植株100株,经PCR技术检测,导入几丁质酶基因和β-1,3葡聚糖酶基因的植株分别为27株和13株,转化率为27%和13%;两个目的基因同时导入的植株10株,转化率为10%。PCR-Southern检测呈阳性,表明外源基因已经整合到番茄基因组中。
New tomato varieties T431, 1911 and 0701 were used as the materials, chitinase gene and β -1, 3-glucanase gene were transformed into tomatoes by Agrobacterium-mediated transformation. A total of 100 regeneration plants were obtained. In these regenerated plants, 27 and 13 plants appeared respectively to be positive in PCR test with chitinase gene and β -1,3-glucanase gene primer. The transferring frequency of foreign gene was 27% and 13% separately. If the two genes were done simultaneously, 10 plants appeared to be positive in PCR test with a transferring frequency of 10%. PCR-Southern analysis of these plants showed that they produced positive hybridization, indicating that the chitinase gene and β -1,3-glucanase gene have been conveyed and integrated into genome of these plants.
出处
《中国农学通报》
CSCD
北大核心
2010年第6期35-38,共4页
Chinese Agricultural Science Bulletin
基金
黑龙江省攻关课题"从碱草中克隆抗逆基因脱宽番茄种质资源的研究"(GA06B103-10)
黑龙江省重大攻关项目"野生五味子驯化
栽培技术"(010-413684)
