摘要
观察了转染Oct-4基因对人脂肪来源干细胞性状的影响.首先将Oct-4基因全序列亚克隆至逆转录病毒载体pMSCVneo中,构建逆转录病毒重组质粒,然后以脂质体介导转染包装细胞PT67,成功建立PT67-Oct4细胞系,随后转染人脂肪来源干细胞(adipose-derived stemcells,ADSCs)中,最后对高表达Oct-4基因的ADSCs性状进行观察.实验结果表明,对高表达Oct-4蛋白的ADSCs-Oct4同未转染Oct-4的ADSCs相比,ADSCs-Oct4在形态学、表面标志、核型等方面无明显差别,接种ADSCs-Oct4细胞8周后裸鼠体内未形成肿瘤.以上结果说明ADSCs-Oct4细胞遗传性状稳定,无致瘤性,为深入开展ADSCs研究提供了实验依据.
The effects of Oct-4 gene transfection on human adipose-derived stem cells （ADSCs） were observed. At first, the whole sequence of Oct-4 gene was sub-cloned into retroviral vector pMSCVneo to construct the recombined retrovirus vector pMSCV-Oct4, and then the recombined plasmid was transferred into packaging cell line PT67 with lipofectarnine. Finally PT-Oct4 cell lines were successfully established. ADSCs were transfected and observed. The results showed that the ADSCs-Oct4 had no obvious difference in morphology, surface marker and karyotype compared to untransfected ADSCs. Furthermore, there was no tumor formed in nude mice 8 weeks after ADSCs-Oct4 inoculation. The above results revealed that the ADSCs-Oct4 had stable genetic traits, no tumorigenicity,and provided the experimental basis for in-depth research.
出处
《河北师范大学学报（自然科学版）》
CAS
北大核心
2010年第2期226-230,共5页
Journal of Hebei Normal University：Natural Science
