摘要
通过在土壤中反复添加不同浓度的土霉素进行预处理,采用平板稀释法测定了土壤细菌在含土霉素培养基及普通培养基上的生长情况,用耐药菌发生率作为指标评价了土霉素污染浓度和污染次数对土壤抗土霉素可培养细菌的影响。结果表明,土霉素污染可诱导土壤土霉素抗性细菌的产生,耐药菌发生率与土霉素添加浓度和添加次数有关。当土霉素添加浓度大于4mg/kg时,一次处理就可诱导土壤可培养细菌产生耐药性;当土霉素浓度为3mg/kg时,二次处理可诱导土壤可培养细菌产生耐药性;当土霉素浓度为2mg/kg时,需要5次处理才可诱导土壤可培养细菌产生耐药性;而当土霉素浓度为1mg/kg时,5次处理均没有明显诱导土壤可培养细菌产生耐药性。研究结果表明,长期施用含土霉素的畜禽粪可导致土壤土霉素抗性细菌的形成和发展。
Antibiotics enter agricultural soils by the use of animal excrements as fertilizers. However,it is scarcely known how these antibiotics affect the cultivable soil bacteria antibiotic resistance. Therefore,effect of antibiotic oxytetracycline （OTC） pollution on cultivable soil bacteria antibiotic resistance was studied by addition of different dose of OTC in the soil. Percentage of soil bacteria antibiotic resistance was evaluated by comparing cultivable bacteria number in culture medium spiked with or without OTC. The results showed that soil OTC pollution could increase the cultivable soil bacteria,which was related to the additional dose and times of OTC. The cultivable soil bacteria antibiotic resistance was significantly increased when soil was treated once with OTC at the dose of 4 mg·kg^-1,or repeatedly treated twice at the dose of 3 mg·kg^-1,or repeatedly treated five times at the dose of 2 mg·kg^-1. But,no significant increase in the cultivable soil bacteria antibiotic resistance was found when soil was treated with OTC at the dose of 1 mg·kg^-1. The results suggested that long-term application of antibiotics-containing animal manure could improve the development of soil bacteria antibiotic resistance.
出处
《浙江农业学报》
CSCD
北大核心
2010年第1期69-72,共4页
Acta Agriculturae Zhejiangensis
基金
高等学校博士学科点专项科研基金(20060335018)
关键词
土霉素
土壤
耐药性
oxytetracycline
soil
antibiotic resistance
