摘要
目的:探讨清毒片联合端粒酶逆转录酶反义核酸(hTERT ASODN)对HL-60细胞增殖及凋亡的影响。方法:体外培养HL-60细胞,分别加入清毒片、hTERT ASODN、清毒片加hTERT ASODN,取培养24、48、72h的细胞,采用四唑盐比色(MTT)法检测细胞增殖、Annexin V-FITC/PI双染流式细胞术检测细胞凋亡。以加入阿糖胞苷组为阳性对照,未处理的细胞为空白对照。结果:清毒片、hTERT ASODN(0.5μmol/L)、阿糖胞苷(10μmol/L)均能抑制HL-60细胞增殖、诱导细胞凋亡,其作用随着培养时间的延长增强;HL-60细胞增殖抑制率及诱导凋亡率从高到低依次为:阿糖胞苷组>清毒片加hTERT ASODN组>hTERT ASODN组>清毒片组;清毒片加hTERT ASODN对HL-60细胞的增殖抑制及诱导凋亡作用明显高于单独使用清毒片或hTERT ASODN(P<0.05)。结论:清毒片与hTERT ASODN联合对于抑制HL-60细胞增殖及诱导凋亡具有协同效应。
Objective：To investigate the effect of Qingdu tablet and hTERT ASODN on the growth and apoptosis of HL-60 cell.Methods：To incubate HL-60 cell with Qingdu tablet,hTERT ASODN,Qingdu tablet add hTERT ASODN,Cytarabine for 24、48、72h. To detect inhibition rate of HL-60 multiplication by MTT and detect apoptosis by Annexin V-FITC/PI double label method with FCM.Results：The QINGDU decoction,hTERT ASODN and Cytarabine all had the effects of inhibiting growth and inducing apoptosis of HL-60 cell.The inhibition ratio of cell growth and apoptotic rate are in following sequence：Cytarabine groupQingdu tablet added hTERT ASODN grouphTERT ASODN groupQingdu tablet group.The effects of inhibiting cell growth and inducing apoptosis of Qingdu added ASODN group was stronger than single Qingdu or ASODN.Conclusion：There are synergistic effects in inhibiting HL-60 cell growth and inducing apoptosis when combined Qingdu tablet with ASODN.
出处
《辽宁中医杂志》
CAS
北大核心
2010年第3期395-398,共4页
Liaoning Journal of Traditional Chinese Medicine
基金
广东省中医药局课题(2007237)
关键词
清毒片
端粒酶逆转录酶反义核酸
HL-60细胞
qingdu tablet
telomerase reverse transcriptase
antisense oligodeoxynecleotide
HL-60 cell
