摘要
应用普通恒场琼脂糖凝胶电泳检测受γ射线照射后小鼠胸腺细胞ＤＮＡ双链断裂（ｄｓｂ）。在一定条件下，该法可以获得与脉冲凝胶电泳相似的结果。用此法观察了离体照射小鼠胸腺细胞ＤＮＡｄｓｂ修复作用，发现该法可以检测到ＤＮＡ“梯状”带，可做为一种研究细胞凋亡的新方法。
It was observed that constant field gel electrophoresis can detect DNA double breaks(dsb) by irradiation. The dsb degree of mice thymocyte by 0～50 Gy (irradiation and the dsb repair in two hours after 30Gy irradiation was studied, the “DNA ladder” was also found using the method.
出处
《辐射研究与辐射工艺学报》
CAS
CSCD
北大核心
1998年第4期234-237,共4页
Journal of Radiation Research and Radiation Processing
基金
国家自然科学基金
关键词
恒场
电泳
Γ射线照射
DNA双链断裂
DSB
Constant field gel electrophoreses,Irradiation, DNA double strand breaks(dsb)
