摘要
采用高效液相色谱法研究恩诺沙星微囊制剂在猪体内各组织中的残留消除规律。恩诺沙星微囊制剂混饲,连续给药7 d,宰杀猪,取组织。组织样品经磷酸盐缓冲液提取,C18固相萃取柱净化,过膜,用流动相:0.05 mol/L磷酸溶液/三乙胺-乙腈(82+18)溶解,微孔过滤,进行HPLC分析。结果表明,当样品浓度为0.02～1μg/g时,线性关系良好。该法最低检测限为20μg/kg,回收率在72%～90%之间,变异系数小于10%。残留在肌肉、脂肪中的恩诺沙星和环丙沙星消除较快,第5天总残留量已下降至检测限以下;肝和肾脏中的残留药物消除较缓慢,第7天肾中药物总残留量为91.48μg/kg。综合各组织中总残留量和MRL规定,建议恩诺沙星微囊制剂休药期为7 d。
The residues depletion of Enrofloxacin（ENR） and Ciprofloxacin（CIP） in the pig tissues was studied by using HPLC method.Enrofloxacin microcapsules was mixed-fed on consecutive seven days.After the last fed,five pigs were slaughtered and collected tissue samples.Tissue samples were extracted by phosphate buffer solution,purified through C18 SPE.Then the extract was dissolved with a mobile phase： 0.05 mol/L phosphoric acid/triethylamine-acetonitrile（82 ＋18）.Finally,the samples were detected by HPLC after filtration.Results showed that,when the sample concentrations were 0.02 μg/g to 1 μg/g,the linear relationship was good.The detection limit was 20 μg/kg.The recovery was between 72% to 90%,and the coefficient of variation was under 10%.The depletion of enrofloxacin in muscle and fat was fast,and the sum of concentration of ENR and CIP was declined,lower than LOD on the 5th day.However,compared with fat and muscle,the depletion of drugs in liver and kidney were slower.The sum of concentration of these in kidney was 91.48 μg/kg at the 7th day.Integrated total residues in tissues,we can identify Enrofloxacin microcapsules withdrawal time is 7 days.
出处
《中国畜牧兽医》
CAS
北大核心
2010年第2期129-131,共3页
China Animal Husbandry & Veterinary Medicine
关键词
恩诺沙星微囊制剂
猪
残留
Enrofloxacin microcapsules
pig
residues
