摘要
根据基因库中单孢子虫的基因保守序列,设计了1对特异性引物,通过对PCR扩增条件的优化,建立了检测贝类单孢子虫的PCR方法。用该方法对单孢子虫模板进行扩增,得到与试验设计相符的244 bp的特异性条带,而对派琴虫、折光马尔太虫、嗜水气单胞菌、荧光假单胞菌、副溶血弧菌、溶藻弧菌、河弧菌和拟态弧菌等病原体的扩增结果全为阴性。敏感性试验结果表明,该方法最低能检测到100 fg的单孢子虫DNA。
According to the gene sequences in GenBank of Haplosporidium sp,a pair of specific primers were designed for amplifying the specific fragments of Haplosporidium sp.The reaction parameters were optimized to develop the polymerase chain reaction method for detection of Haplosporidium sp.The 244 bp long specific DNA fragment of Haplosporidium sp was amplified,but not from other pathogenic such as Perkinsus sp,Marteilia refringens,Aeromonas hydrophila,Pseudomonas fluorescens,Vibrio parahaemolyticu,Vibrio Alginolyticu,Vibrio Fluvialis and Vibrio Mimicus by PCR.The sensitivity results showed that as little as 100 fg DNA of Haplosporidium sp was detected by this PCR.
出处
《中国畜牧兽医》
CAS
北大核心
2010年第1期54-56,共3页
China Animal Husbandry & Veterinary Medicine
基金
国家百千万人才工程人选专项资金项目(945200603)
广西科技攻关项目(桂科攻0630001-3M)共同资助
