摘要
本文用一对引物对我国６个地区的银鲫进行ｍｔＤＮＡ－ＮＤ５／６片段的ＰＣＲ扩增，扩增产物（约２．５Ｋｂ）用６种限制性内切酶进行限制性片段长度多态性（ＲＦＬＰ）分析。共获得１１种图谱和７种限制性类型（基因型）。根据各群体间的遗传距离构建了ＵＰＧ聚类关系图。结果表明，６个银鲫种群中，云南群体与贵州群体的关系以及黑龙江与安徽群体之间的关系最近，它们分别归为一个群体；河南群体相对独立，它与黑龙江和安徽群体的遗传关系较近；江西群体与各群体间的亲缘关系最远。
In six geographical populations of Carassius auratus gibelio, the ND 5/6 region (approximately 2.5Kb) of mtDNA genome have been amplified by the polymerase chain reaction (PCR), and the products were examined using restriction fragment length polymorphism (RFLP). 11 electrophoresis patterns and 7 haplotypes (genotypes) have been observed. Based on the genetic distances, the UPG molecular phylogenetic tree of the populations was constructed. The results indicate that the six populations of the C. auratus gibelio can be imcorporated into four populations, namely, the Heilongjiang and Anhui, the Henan, the Yunnan and Guizhou, and the Jiangxi.The first is the closest to the third. The Henan is much closer to the Heilongjiang and Anhui. The farthest is between the Jiangxi and the other populations.
出处
《水产学报》
CAS
CSCD
北大核心
1998年第4期289-295,共7页
Journal of Fisheries of China
基金
"九五"农业部重点科研项目
