摘要
在用牛肺巨噬细胞构建了ｃＤＮＡ基因文库的基础上，参照人和小白鼠ＣＤ１４的基因序列设计一对引物，用ＰＣＲ方法成功地扩增出编码牛ＣＤ１４的特异性ＤＮＡ片段，并进行了序列分析。结果表明，牛ＣＤ１４基因的核苷酸在相同区域内与人ＣＤ１４的同源性为７９％，推导氨基酸序列的同源性为７３％；而与小白鼠的核苷酸和氨基酸的同源性分别为７５％和７１％。无论在核苷酸还是氨基酸水平上，牛ＣＤ１４基因与人ＣＤ１４的同源性都要高于与小白鼠的同源性。
On the base of established cDNA gene library of bovine alveolar macrophages, a pair of primers were designed according to the gene sequences of human and murine CD14 antigens, and then the specifical DNA fragment encoding bovine CD14 antigen was successfully amplified and sequenced by means of PCR. The test results showed that the identities of nucleotides and the deduced amino acids were 79% and 73% between cattle and human, and 75% and 71% between cattle and mouse, respectively, which indicated the gene structure of bovine CD14 antigen is more closely related to human's than that of mouse.
出处
《中国兽医科技》
CSCD
1998年第12期12-14,共3页
Chinese Journal of Veterinary Science and Technology
