摘要
台湾番茄曲叶病毒(Tomato leafcurl Taiwan virus,ToLCTWV)近年来在我国部分番茄种植区流行成灾,对番茄生产造成了严重损失。作者研究了B型烟粉虱对ToLCTWV的获取、保持,并测定了虫口密度、温度及植株苗龄对其传播该病毒的影响。结果表明,B型烟粉虱在感染ToLCTWV的番茄植株上取食0.5h即可在3.3%个体内检测到ToLCTWV DNA,取食48h后,带毒率达100%;ToLCTWV DNA可在B型烟粉虱体内终生存留。B型烟粉虱是ToLCTWV的高效媒介,每株1头带毒烟粉虱取食48h,植株发病率达50%～60%;每株5头取食,发病率达到或接近100%。介体虫口密度及植株苗龄是决定苗期发病程度的重要因子,虫口密度增加,病情指数上升;苗龄增大,病情指数下降。温度对发病率有显著影响,但对病情指数影响不显著。此外,温度与苗龄的互作、虫口密度与苗龄的互作以及三因子之间的互作都对病情指数有显著影响。这些结果提示,培育无病壮苗是防治台湾番茄曲叶病毒病的关键。
In recent years Tomato leaf curl Taiwan virus （ToLCTWV） has caused disease epidemics and heavy losses to crop production in several tomato-growing regions in China. To provide basic information for the control of the disease, we investigated the capacity of the B biotype Bemisia tabaci to acquire and retain ToLCTWV, as well as the effects of vector density, plant age, and temperature on the transmission of ToLCTWV by the whiteflies. ToLCTWV DNA was detected in 3.3% of the whiteflies when they had fed for 0.5 h on ToLCTWV-infected tomato plants and was detected in 100% of the whiteflies when the acquisition access period increased to 48 h. Viruliferous whitefly adults retained ToLCTWV DNA for their entire life. Our data showed that the B biotype whitefly was an efficient vector for ToLCTWV. Transmission was achieved to 50% -60% of the plants by one adult per plant, and the frequency of transmission approached or reached 100% when the vector density increased to five per plant. Vector density and plant age are two main factors affecting disease severity of tomato seedlings, which increased with vector density but decreased＇ with plant age, respectively. Temperature had a significant effect on the incidence of disease, but no significant effect on disease severity. In addition, the interactions between temperature and plant age, between vector density and plant age as well as the interactions between the three factors all had significant effects on the disease index. These results suggested that cultivating virus-free and healthy seedlings is of vital importance in the control of ToLCTWV diseases in the field.
出处
《植物保护学报》
CAS
CSCD
北大核心
2010年第1期1-6,共6页
Journal of Plant Protection
基金
浙江省重大科技专项(优先主题)农业项目(2007C12054)
国家科技支撑计划课题(2006BAD08A18)
