摘要
以优选果梅黔荔1号单株为材料,对外植体灭菌时间、腋芽启动和增殖培养进行了研究。结果表明:以0.1%HgCl_2消毒8 min效果最好,其存活率为75%;适合腋芽启动培养的基本培养基是WPM;最有利于腋芽启动和增殖的培养基是WPM+1.0 mg/L6-BA+0.5 mg/LNAA+1.0 mg/LGA_3,有效增殖系数高达1.51。
Using better single plant （Qian Li NO. 1） from Prunus mume as materials,explants sterilization, and proliferation culture of axitlary buds were studied. The results showed that the optimal sterilization time of explant was about 8 minutes with 0. 1G HgCl2 ,and its survival rate was 75G. The basal medium of of axillary buds was V, PM. The best medium for and proliferation of axillary buds was WPM＋1. 0 mg/L 6-BA＋0. 5 mg/L NAA＋1. 0 mg/L GA3 ,and its effective muhiplication coefficient was 1.51.
出处
《北方园艺》
CAS
北大核心
2010年第3期118-120,共3页
Northern Horticulture
基金
贵州省科技攻关资助项目(黔科合NY字[2007]3039号)
贵州省省长基金资助项目(黔省专合字2007-18号)
贵州省果树学科科技创新人才团队建设资助项目(黔科合人才团队[2008]88007号)
贵州省特色农业产业人才培养基地建设资助项目
贵州省果树工程技术研究中心建设资助项目(黔科合农G字[2007]4001号)
关键词
果梅
腋芽
离体繁殖
生长调节剂
Prunus mume
axillary bud
in vitro propagation
plant growth regulator
