摘要
探索了大葱花药处于单核晚期的形态指标,并以B5为基本培养基,分别添加2,4-D(2.0～3.0 mg/L)、6-BA(0.5 mg/L)、谷胱甘肽(800 mg/L)等,对大葱花药进行了离体培养。结果表明:32℃热击处理有利于大葱花药诱导愈伤组织;培养集中添加6%浓度的蔗糖,有助于大葱愈伤组织的培养;大葱愈伤组织诱导率较低,且不易分化。
The cytological observations of pollen in different developmental stages of welsh onion were undertook,135＋2, 4-D（2.0 = 3.0 mg/L） ＋ 6- BA（0. 5 mg/L） ＋ glutathione （800 mg/L） was used for callas formation. The results showed that pretreatment of anther after inoculation at 32℃ for 3 d and the medium with 6 % sucrose were favorable for callus formation. The callus induction rate of anther was low,and its differentiation was not easy.
出处
《北方园艺》
CAS
北大核心
2010年第3期113-114,共2页
Northern Horticulture
关键词
大葱
花药培养
愈伤组织
welsh onion
anther cullture
calls
