摘要
目的分析近年来宁波市乙型流行性感冒(流感),两大谱系毒株流行情况和病毒血凝素基因特性。方法用犬肾传代细胞(MDCK)分离流感病毒,对2003-2009年分离的乙型流感病毒按不同的谱系选择每年的代表株,经聚合酶链反应扩增目的基因后进行乙型流感病毒的血凝素基因HA1区域(HA1)核苷酸序列测定。结果乙型流感两大谱系的病毒在宁波市几乎每年均有出现,相互交替成为优势株。其中Yamagata系在2004及2007年相对占优势,而Victoria系在2006年3月及2009年6月2次出现了流行高峰。HA1序列测定结果Yamagata系毒株在2003、2007年HA1区出现2次出现明显的氨基酸替换,Victoria系在2005年底及2009年初发生2次明显氨基酸替换。结论2003-2009年乙型流感的两大谱系病毒的HA1序列均存在较大差异,本市2004及2007年Yamagata系2次在乙型流感中相对占优势和2006及2009年Victoria系出现的2次乙型流感流行高峰均与病毒HA1区的氨基酸替换有关。
Objective To understand the prevalence and genetic characteristics of two lineages of influenza B viruses isolated recently in Ningbo. Methods Influenza viruses were isolated with madin-darby Canine Kidney （MDCK） cell, and the representative strains were selected according to the lineages of influenza B viruses isolated during 2003-2009, the target genes were amplified by reverse transcriptase polymerase chain reaction （RT-PCR）, the product was purified and determined for nucleotide sequence of HA1 region of the haemagglutinin gene of influenza B viruses. Results During 2003-2009, two lineages of influenza B viruses co-circulated in Ningbo nearly every year which were predominant alternatively. Yamagata lineage virus predominated in 2004 and 2007 relatively, but Victoria lineage virus was highly prevalent in March, 2006 and in June, 2009. The HA1 region sequencing indicated the obvious amino acid substitution for Yamagata lineage virus in 2004 and 2007 and for Victoria lineage vires in 2005 and 2009. Conclusion During 2003-2009, the haemagglutinin genes of two lineages of influenza B viruses had obvious difference. The predominance of Yamagata lineage virus in 2004 and in 2007 and the high prevalence of Victoria lineage virus in 2006 and 2009 in Ningbo were related to the amino acid substitution of HA1 region of influenza B viruses.
出处
《疾病监测》
CAS
2010年第1期33-36,42,共5页
Disease Surveillance
