摘要
目的研究甲基强的松龙对大鼠脊髓损伤后微管相关蛋白-2(MAP-2)表达的影响。方法健康成年Wistar大鼠66只,随机取6只作为正常对照组,60只制作成脊髓打击伤动物模型。模型大鼠随机分为甲基强的松龙组和治疗对照组,每组30只,分别于伤后1、3、7、14和28 d取材,应用免疫组织化学方法观察MAP-2的表达,用改良的Tarlov评分观察大鼠脊髓损伤后运动功能的恢复情况。结果大鼠脊髓损伤后7和14 d,甲基强的松龙组MAP-2的表达强于治疗对照组;大鼠脊髓损伤后7、14和28 d,甲基强的松龙组Tarlov评分大于治疗对照组。结论甲基强的松龙能促进大鼠损伤脊髓表达MAP-2,并能促进大鼠脊髓损伤后运动功能的恢复。
Objective To investigate the effects of methylprednisolone on the expression of microtubule associated protein-2 （MAP-2） after spinal cord injury in rats. Methods Six rats were selected randomly from 66 adult healthy Wistar rats as the normal control group, and the rest was divided into two groups after contusion injury was performed： methylprednisolone group and treatment control group, 30 rats for each group. On the day 1, 3, 7, 14 and 28 after injury, the rats were killed, the expression of MAP-2 was detected with immunohistochemistry. The recovery of motor function after spinal cord injury was assessed with improved Tarlov scores. Results The expression of MAP-2 was higher in methylprednisolone group than that in treatment control group on the day 7 and 14 after spinal cord injury in rats. Improved Tarlov scores in methylprednisolone group were higher than that in treatment control group on the day 7, 14 and 28 after spinal cord injury in rats. Conclusion Methylprednisolone can promote the expression of MAP-2 in spinal cord and the recovery of motor function after spinal cord injury in rats.
出处
《兰州大学学报（医学版）》
CAS
2010年第1期1-4,共4页
Journal of Lanzhou University（Medical Sciences）
关键词
脊髓损伤
甲基强的松龙
微管相关蛋白-2
spinal cord injury
methylprednisolone
microtubule associated protein-2
