摘要
背景与目的:近年关于肝癌干细胞问题备受关注,已发现部分干细胞标志物在肝细胞癌中表达并与预后有关,其中OCT4基因是POU转录因子的重要组员,是维持胚胎干细胞自我更新能力和分化潜能的关键因子。本研究旨在检测OCT4A在肝细胞癌中的表达情况,并探讨其与病理因素和预后的关系。方法:用半定量RT-PCR检测5株人肝癌细胞系和1株永生化肝细胞系,以及107例肝细胞癌组织、相应的癌旁肝组织和20例正常无肝硬化肝组织中OCT4A mRNA的表达,并结合肝癌临床病理因素及预后进行分析。结果:OCT4A mRNA在人肝癌细胞系SMMC-7721、BEL-7402、Hep-G2、MHCC-97L、MHCC-97H中均表达,而在永生化肝细胞系L-02中无表达。OCT4A mRNA在肝癌组织中表达率为72.0%,明显高于癌旁肝组织的30.8%(P<0.001),在正常无肝硬化肝组织中无表达。肝癌组织中OCT4A mRNA的表达与肿瘤大小、血管侵犯及TNM分期有关(P<0.05)。OCT4A mRNA阳性肝癌患者及阴性患者的3年总生存率分别为48.0%和83.7%,3年无瘤生存率分别为34.1%和58.0%,差异均有统计学意义(P<0.05)。结论:OCT4A mRNA在肝癌细胞及肝癌组织中高表达,可能在肝癌发生发展中发挥重要作用,可以作为肝癌临床预后的一个重要参考指标。
Background and Objective：Recently,many studies have focused on stem cells in hepatocellular carcinoma （HCC） and found some stem cell markers in HCC,which are associated with the prognosis of HCC.OCT4,as a member of the POU transcription factor family,is a key factor to maintain self-renewal and pluripotency of embryonic stem cells （ESCs）.This study was to explore the expression of OCT4A in HCC and its correlation with clinicopathologic features and prognosis of HCC.Methods：OCT4A mRNA expression was detected in 5 liver cancer cell lines （SMMC-7721,BEL-7402,Hep-G2,MHCC97-L,and MHCC97-H）,1 immortalized liver cell line （L-02）,107 pairs of samples of HCC tissues and matched non-neoplastic liver tissues,and 20 samples of normal liver tissues using semi-quantitative reverse transcription-polymerase chain reaction （RT-PCR）.The correlation of OCT4A mRNA with clinicopathologic features and prognosis of HCC was analyzed.Results：OCT4A mRNA was detected in SMMC-7721,BEL-7402,Hep-G2,MHCC-97L,and MHC...更多C-97H,but not in L-02.The positive rate of OCT4A mRNA expression was significantly higher in the HCC tissues than in the nonneoplastic liver tissues （72.0% vs.30.8%,P〈0.001）.OCT4A mRNA expression was not detected in the normal liver tissues.OCT4A mRNA expression was correlated with the tumor size,vascular invasion,and TNM stage （P〈0.05）.Kaplan-Meier survival curves showed that patients with positive expression of OCT4A mRNA had lower overall survival rate and disease-free survival rate.Conclusions：OCT4A mRNA,which was highly expressed in a subset of liver cancer cell lines and HCC tissues,may be involved in carcinogenesis of HCC.OCT4A mRNA may be a valuable biomarker for assessing prognosis of HCC.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
2010年第1期111-116,共6页
Chinese Journal of Cancer
基金
国家自然科学基金项目(No.30872489)~~
