摘要
采用ｐｃＤＮＡ３与ｐＭＴ两种基因表达裁体，构建包含丙型肝炎病毒（ＨＣＶ）核心区（Ｃ区）或全结构基因区（核心区、包膜区）的质粒。经纯化后直接对Ｂａｌｂ／ｃ小鼠进行骨骼肌或尾部皮内注射，２～６ｗ后采血，用ＥＬＩＳＡ法检测小鼠抗核壳区的体液应答。结果表明：ＤＮＡ免疫可引起小鼠产生抗ＨＣＶ核壳区的体液应答，加强免疫２次后可使特异性抗体平均滴度达到１８００以上。提示ＤＮＡ免疫可作为丙型肝炎病毒感染的潜在防御手段。
Plasmids which contains hepatitis C virus nucleocapsid region alone or structural gene were constructed by cloning into two eukaryotic expression vector(pcDNA3,pMT).Intramuscular or intradermal immunization of Balb/c mice with these constructs in the form of naked DNA elicited humoral responses to the HCV nucleocapsid within 2 to 6 weeks postinjection in enzyme linked immunosorbent assay.The mean serum titer anti nucleocapsid specific antibody were found to be above 1∶800 after two boosted injections,and these titers remained stable until the end of the study(18 weeks postinjection).This findings suggest the HCV structural gene immunization warrants further investigation as a potential vaccine against HCV infection.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1998年第6期453-455,共3页
Chinese Journal of Immunology
基金
中国预防医学科学院病毒学研究基金
关键词
丙型肝炎病毒
DNA免疫
体液应答
Hepatitis C virus DNA based immunization Humoral response
