摘要
研究观察了Ｍ－ＣＳＦ和ＩＦＮ－γ基因转染的巨噬细胞（Ｍａｃｒｏｐｈａｇｅ，Ｍφ）的体外抗原提呈能力，并对其机制进行了初步探讨，结果表明，ＩＦＮ－γ基因转染及Ｍ－ＣＳＦ／ＩＦＮ－γ联合基因转染的Ｍφ抗原提呈功能显著增强，联合基因转染组优于单基因转染组（Ｐ＜０．０５），Ｍφ表面Ｉａ、Ｂ７－１、ＩＣＡＭ－１的表达水平有不同程度的提高，这些细胞表面相关分子的高表达可能与其抗原提呈能力增加有关。混合淋巴细胞反应结果提示，当用５倍于Ｍφ量的肿瘤细胞制备的肿瘤抗原触发４ｈ，Ｔ淋巴细胞的增殖能力最强，该结果为过继回输肿瘤抗原体外触发的基因转染的Ｍφ治疗肿瘤提供了实验依据。
In the present study,the antigen presenting ability of M CSF and IFN γ gene cotransfected macrophages were observed and their mechanisms were investigated.The results showed that the antigen presenting ability of macrophages transfected with IFN γ gene or M CSF and IFN γ gene co transfected were enhanced significantly and that of cotransfected group were much higher than gene transfection alone(P<0.05).Ia,B7 1 and ICAM 1 expression on the surface of Mφ were increased,this might related to the increased antigen presenting ability.Results of MLR indicated that when gene transfected Mφ pulsed with tumor antigen(obtained from tumor cells 5 time of Mφs for 4 hours,the proliferation ability of T lymphocytes were strongest.The results supplied the experimental bases for tumor therapy using adoptive infusion of gene transfected Mφ pulsed with tumor antigen.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
1998年第6期399-402,共4页
Chinese Journal of Immunology
基金
国家"863"九五项目
关键词
巨噬细胞
M-CSF
IFN-Γ
基因转染
抗原提呈
肿瘤
Macrophage colony stimulating factor
Interferon gamma
Gene transfection
Macrophage
Antigen presenting
