摘要
目的：研究川芎嗪对大鼠脑缺血再灌注损伤后Bcl-2表达的影响。方法：以线栓法制作大鼠大脑中动脉阻塞的局灶性脑缺血再灌注模型，采用免疫印迹、2，3，5-氯化三苯四氮唑（TTC）、H—E染色和神经行为相结合的方法，观测缺血再灌注侧大脑皮质内Bcl-2的表达、脑梗塞体积、脑组织结构及神经功能的变化。结果：与缺血再灌注组（I2h／R24h）比较，川芎嗪组的Bcl-2表达明显增高，脑梗塞体明显缩小，脑组织的病理损伤和神经异常行为明显减轻。结论：川芎嗪可通过上调Bcl-2的表达，缩小脑梗塞的体积和减轻脑缺血区组织结构的损伤以及明显改善神经症状，对脑缺血再灌注损伤有保护作用。
Objective： To investigate the effect of tetramethylpyrazine on the expression of Bcl-2 after cerebral ischemic-reper- fusion injury. Methods： The focal cerebral ischemic-reperfusion model was established with thread embolism of the middle cerebral artery. Immunohistochemistry, TTC staining, H-E staining and neurological evaluation were used to examine the expression of Bcl-2 in the cerebral cortex, cerebral infarction volume, pathological damage of brain tissue and neurological function disorders. Results： Compared with the ischemic-reperfusion model group （I2 h/R24 h）, the content of Bcl-2 was in- creased significantly, cerebral infarction volume and neurological function disorders were markedly downregulated, and the pathological damage of brain tissue was attenuated in tetramethyleyrazine treatment group. Conclusion： Tetramethyleyrazine has a neuropretective effect by increasing the expression of Bcl-2, downregulating cerebral infarction volume, attenuating the pathological damage of brain tissue and improving neurological symptoms.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2010年第1期82-85,共4页
Chinese Journal of Anatomy
基金
山西省高校科技研究开发项目（200613049）
关键词
脑缺血再灌注
川芎嗪
BCL-2
大脑皮质
大鼠
cerebral ischemic-reperfusion
tetramethylpyrazine
Bcl-2
cerebral cortex
rat
