摘要
目的观察S100钙结合蛋白A8(S100A8)和S100钙结合蛋白A9(S100A9)在细胞内定位及脂多糖(lipopolysaccharide,LPS)刺激对其细胞内定位的影响。方法将带有血凝素(HA)标签的S100A8或S100A9真核表达载体瞬时转染至小鼠Hepa1-6肝癌细胞,通过细胞免疫荧光方法观察S100A8、S100A9的胞内表达、定位及LPS对其定位的影响。结果静息状态下,S100A8、S100A9在细胞浆和细胞核都有分布;LPS刺激细胞2h后,S100A8移位入核,而S100A9在细胞内分布无明显变化。结论LPS刺激后S100A8、S100A9在细胞内的差异分布,提示两者在炎症反应中的作用不同,S100A8可能参与LPS诱导的基因表达调控。
Objective To observe the location of S100A8 and S100A9 in Hepa 1-6 cells and the effect of lipopolysaccharide （LPS） on the location.Methods The eukaryotic expression vectors of S100A8 and S100A9 with HA tag were transiently transfected into Hepa 1-6 cells,then subcellular location of S100A8 and S100A9 and the effect of lipopolysaccharide （LPS） on the location was detected by immunofluorescence.Results S100A8 and S100A9 were distributed in the cytoplasm and nuclei at rest.2 h after LPS treatment cells,S100A8 markedly translocated into nuclei,but the distribution of S100A9 in cells had no significant change.Conclusion The differential distribution of S100A8 and S100A9 indicates they play a different role in inflammation.S100A8 probably participated in the expression regulation of genes induced by LPS.
出处
《中国现代医药杂志》
2010年第2期1-3,共3页
Modern Medicine Journal of China
基金
国家自然科学基金面上项目(NO.30670828)
