摘要
目的:从观察脑组织谷氨酸(GLU)、天门冬氨酸(ASP)、甘氨酸(GLY)含量和核转录因子(NF-κB)表达的变化,揭示针刺穴位抗老年脑缺血再灌注I/R损伤的保护机制。方法:采用大脑中动脉闭塞方法造成老龄大鼠脑缺血动物模型,大鼠随机分组为假手术组、模型组、非穴位组、穴位治疗组。每组又根据缺血及再灌注时间不同分为缺血3h后再灌注1、3、7、12d4个时间点观察。观察各时间点大鼠神经症状积分、脑组织含水量、病理变化、高效液相色谱法测定脑组织GLU、ASP、GLY;免疫组织化学方法测定脑组织NF-κB表达。结果:与假手术组比较,模型组各时间点大鼠神经症状积分、脑组织含水量、GLU、ASP含量和NF-κB表达明显升高,GLY含量无明显变化。与模型组比较,穴位治疗组各时间点大鼠神经症状积分、脑组织含水量、GLU、ASP含量和NF-κB表达显著降低(P<0.05),GLY含量无明显变化;与模型组比较,非穴位组各时间点大鼠神经症状积分、脑组织含水量、GLY、GLU、ASP含量和NF-κB表达无明显变化。结论:脑缺血可增强大鼠脑组织兴奋性氨基酸释放和NF-κB表达;针刺穴位抗脑缺血再灌注损伤的机制与其降低脑组织兴奋性氨基酸神经毒性和抑制NF-κB表达有关。
Objective： To study the protective effect of electro-acupuncture on glutamate（GLU）, glycine（GLY）, aspartate （ASP） and NF-κB expression in rat with cerebral ischemia reperfusion. Methods： The middle artery occlusion （MCAO） model was established and high pressure liquid chromatography （HPLC） fluorescence assay was used to detect contents of glutamate （GLU）, glycine （GLY） and aspartate （ASP） in rat brain. The effect of electro-acupuncture on the nervous dysfunction score, the water content of cerebral constitution and the expression of NF-κB were observed. The group with non-acupoint group was as control. Results： The nervous dysfunction score, the water content of cerebral constitution, NF-κB, GLUand ASP in model group were higher than those of the sham-operated group. GLY levels had no obvious changes, there was no significant diference between the model group and the non-acupoint group. The nervous dysfunction score, the water content of cerebral constitution,the expression of NF-κB, GLU and ASP in electro-acupuncture group were lighter than that of the model group, GLY levels had no obvious changes. Conclusion： Electro-acupuncture could protect brain cell from damage after focal cerebral ischemia reperfusion injury by inhibiting the cerebral expression of NF-xB and excitatory amino acid.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2010年第3期462-465,共4页
China Journal of Traditional Chinese Medicine and Pharmacy
