摘要
采用框栽试验方法,研究一次供氮(指在总氮量一定的情况下以基肥的形式一次性施入,设3种总供氮量处理:75 kg/hm2、150 kg/hm2、225 kg/hm2)和分次供氮(指在总氮量一定的情况下,分别于播种前、苗期、花期和鼓粒期分4次平均施入,与一次供氮处理相同,设3种总供氮量处理)对大豆根瘤干重和数量、氮积累量、固氮酶活性及豆血红蛋白含量的影响。结果表明:一次性施氮方式下,各处理地上氮积累量随着施氮量的增加而增加,根系氮积累量则随着施氮量的增加呈先增加后下降的趋势。分次施氮方式下,花期和鼓粒期根瘤干重和数量的变化趋势一致;苗期,各组织氮积累均以一次性施氮方式高于分次施氮和对照,且随着施氮量的增加而增加(地上);随着生育时期的推进,分次施氮对大豆植株氮积累促进作用增大,各组织氮积累表现为分次施氮>一次性施氮>对照。施氮量对固氮酶和豆血红蛋白含量的影响与一次性施氮方式下相似,但整体上较之抑制作用更强。
A pot culture experiment was carried out to study the effects of one-off nitrogen supply （ ONS, i. e. the fertilizer was applied once as base fertilizer at three levels of 75 kg/hm^2, 150 kg/hm^2 ,225 kg/hm^2） and discrete nitrogen supply（ DNS, i. e. all the fertilizer was divided into four equal parts, one of which was then applied at the stages of before sowing, seedling, flowering and podding, respectively, at the same rates as those of ONS） on the variation of nodule dry weight （DW）, nodule number, N accumulation, nitrogenase activity and leghemoglobin （Lb） concentration in the nodule of soybean （Glycine max L. ）. Under ONS, the shoot N accumulation increased with the increment of N application rate, however, the root N accumulation showed another trend of increasing before decreasing. Under the DNS, the nodule DW and number at flowering and podding stages showed the same trend. The N accumulation of ONS was higher than that of DNS and CK, and increased with the N application rate of shoot. Besides, the DNS accelerated the N accumulation of soybean. ONS and DNS had the same effects on nitrogenase activity and Lb concentration, but the ONS exhibited stronger inhibition.
出处
《江苏农业学报》
CSCD
北大核心
2010年第1期75-79,共5页
Jiangsu Journal of Agricultural Sciences
基金
国家科技支撑计划项目(2006BAD21B01
2006BAD05B05)
黑龙江省科技攻关项目(GB06B101-1-4)
关键词
根瘤
固氮
供氮方式
大豆
nodule
nitrogen fixation
nitrogen supply method
soybean
