摘要
目的通过分析PTEN、E-cd和MMP-9在人脑胶质瘤中的表达，为胶质瘤的靶分子治疗提供依据。方珐采用免疫组织化学方法检测50例人脑胶质瘤和11例正常脑组织中的PTEN、E—cd蛋白和MMP-9的表达。分析人脑胶质瘤组织学分级之间的PTEN、E—cd蛋白和MMP-9的表达关系及胶质瘤与正常脑组织之间的PTEN、E-cd蛋白和MMP．9的表达关系，并探讨其临床意义。结果PrrEN、E—cd在正常脑组织中的阳性表达为100％，VIEN在Ⅰ-Ⅱ级和Ⅲ-Ⅳ级胶质瘤中阳性表达率分别为75％和38％，组织学分级之间有显著差异（P〈0．05），与对照组之间差异有统计学意义（P〈0．05）；E—cd在Ⅰ-Ⅱ级和Ⅲ-Ⅳ级胶质瘤中阳性表达率分别为42％和23％，组织学分级之间差异无统计学意义（P〉0．05），与对照组之间差异有统计学意义（P〈0．05）；MMP-9在对照组阳性表达率为14％，在脑胶质瘤Ⅰ-Ⅱ级为33％，Ⅲ-Ⅳ级为62％，两者比较差畀有统计学意义（P〈0．05）；对照组与神经胶质瘤组相比，MMP-9表达差异有统计学意义（P〈0．05）。结论PTEN、E—cd、MMP-9在神经胶质瘤的生物学行为中起重要作用，且可能与其侵袭性有关。
Objective To investigate expressions of tumor suppressor PTEN, E-cadherin（E-cd） and matrix metalloproteinase-9（ MMP-9 ）in human glioma and to elucidate its clinical significance. Method Immunohistochemical staining was used to detect expressions of PTEN, E-cd and MMP-9 in 50 cases of human glioma and 11 of normal brain tissues. Results In normal brain tissues, the positive rates of E-cd and PTEN were 100% and of MMP-9 was 14%. The positive rate of PTEN in glioma was lower than that in the control group（P 〈0. 05）, and the positive rate of PTEN in low grade glioma was higher than that in high grade glioma （75% vs. 38%, P 〈0. 05）. The positive rate of E-cd in glioma was lower than that in the control group （ P 〈 0. 05 ）, but there was no significant difference between low grade glioma and high grade glioma （42% vs. 23% , P 〉0. 05）. The positive rate of MMP-9 in glioma was higher than that in the control group（ P 〈0.05 ）, and there was significant difference between low grade glioma and high grade glioma （33% vs. 62%, P 〈0. 05 ）. Conclusion PTEN, E-cd and MMP-9 play important roles in the biological behavior of glioma, which may be related to invasiveness.
出处
《山东大学学报（医学版）》
CAS
北大核心
2010年第2期109-112,共4页
Journal of Shandong University:Health Sciences
