摘要
采用盆栽试验，利用１０ｍｍｏｌ／Ｌ 的Ｈ２Ｏ２ 对冷季型草坪草高羊茅进行叶面喷施处理，研究外源低浓度Ｈ２Ｏ２对高羊茅叶片中抗氧化系统的调控作用及其对高羊茅抗热性的影响。结果表明，Ｈ２Ｏ２ 可能作为信号分子预先增加抗氧化酶的活性，改变抗氧化剂的浓度，从而减轻随后发生的热胁迫对草坪草造成的氧化伤害；在胁迫过程中ＰＯＤ 和ＣＡＴ 活性在Ｈ２Ｏ２ 预处理后增加不显著，热胁迫本身增加了ＰＯＤ 的活性，但是降低了ＣＡＴ 活性，ＰＯＤ 对于提高高羊茅的耐热性可能具有更重要的作用；外源Ｈ２Ｏ２ 显著影响了高羊茅叶片中的ＡｓＡ－ＧＳＨ 循环，其中处理植株中的ＡＰＸ、ＧＰＸ 和ＧＲ 的活性在热胁迫过程中增加２０％ ～１１０％，ＧＳＨ／ＧＳＳＧ 下降了８０％，与高羊茅抗热性的提高密切相关。可见信号分子Ｈ２Ｏ２ 可以通过调控高羊茅的抗氧化系统提高其抗热性。
Cool-season turfgrass Festuca arundinacea was pretreated with 10 mmol/L H2 O2 in pot experiments, and the regulation of the antioxidative system by exogenous H2O2 and its effects on heat-tolerance were stud ied. Exogenous H2O2 may activate the defensive system in F. arundinacea ahead of heat stress, and alleviate oxidative damage resulting from subsequent heat stress. increased by exogenous H2O2 treatment. Heat stress itself important role in improving heat resistance of the turfgrass CAT and POD activities were not significantly increased POD activity, and POD may play a more H2O2 significantly affected the AsA--GSH cycle in F. arundinacea. APX, GPX and GR activities increased 20%--110% and GSH/GSSG significantly decreased 80% in signalin the cycle, and were closely associated with the acquirement of heat tolerance in F. arundinacea. The g molecule H2O2 can improve the heat-tolerance of F. arundinacea by regulating the antioxidative sys tern.
出处
《草业学报》
CSCD
北大核心
2010年第1期89-94,共6页
Acta Prataculturae Sinica
基金
国家高科技研究发展计划(863计划)项目(2007AA10Z231)
南京大学博士后科研启动基金(0208003089)资助
