摘要
以2种类型北美红杉(观赏型和用材型)的嫩枝为外植体,通过植株再生与生根条件的研究,解决北美红杉生根的关键问题,建立起标准化的离体繁殖技术体系。结果表明:北美红杉是一种较易增殖的木本植物,2种类型增殖的适宜培养基均是MS+6-BA0.1mg/L+NAA0.1mg/L+KT0.1mg/L;无机盐浓度是其生根的关键因子,基本培养基所有成分降为原来的1/4时,生根快且齐,生根的最佳培养基是1/4MS+NAA0.4mg/L,但观赏型北美红杉较用材型生根效果好。
Take two types（watching type and wooding type）of softwood as explants,by studying the plant regeneration and rooting condition,to establish standardized in vitro propagation technology system for Sequoia sempervirens（Larnb.）Endl.The results showed that Sequoia sempervirens（Larnb.）Endl was a kind of woody plant multiplication easily and the inorganic salt concentration was the key factor for its rooting;their optimal multiplication medium was MS＋6-BA 0.1 mg/L＋NAA 0.1 mg/L＋KT0.1 mg/L,and the best rooting medium was 1/4MS＋NAA 0.4 mg/L,the watching type rooting better than the wooding type.
出处
《中国农学通报》
CSCD
北大核心
2010年第4期89-92,共4页
Chinese Agricultural Science Bulletin
基金
云南省科技厅"云南省人才培引项目"(2006PY04)
关键词
北美红杉
嫩枝
标准化离体繁殖
Sequoia sempervirens（Larnb.）Endl softwood standardized technology of in vitro propagation
