摘要
农药残留是影响茶叶质量安全的重要因素,为了建立茶叶中甲氰菊酯和溴氰菊酯残留量快速检测方法,以乙腈为提取溶剂,采用机械振荡提取,提取液经活性炭固相萃取柱和中性氧化铝固相萃取柱串联净化后,带状点样至硅胶60F_(254)高效薄层板上,以甲苯为展开剂展开后用Camag TLC Scabber3在230 nm处扫描。结果表明,甲氰菊酯(Rf=0.49)和溴氰菊酯(Rf=0.64)的最小检测限分别为20.0 ng和10.0 ng。茶叶中甲氰菊酯和溴氰菊酯的添加回收率为94.8%～108%和90.5%～102%(添加质量浓度0.50～10.0 mg/kg),相对标准偏差为1.71%～6.22%和3.82%～6.39%。该试验所建立方法的准确性、精确性均满足农药残留分析的要求。
Pesticide residue is an important factor to impact on quality safety of tea.In order to set up a rapid determination method of fenpropathrin and deltamthrin residues in tea,in this paper,acetonitrile as extract solvent,extracted by mechanical vibration and cleaned up by a Carb and Alumina-N tandem SPE column.The extract was applied as bands to the silica gel 60F254 HPTLC plates.The plates were developed with toluene,and scanned with a Camag TLC Scanner3.The results indicated that the detection limits of fenpropathrin（Rf=0.49） and deltamethrin（Rf=0.64） were 20ng and 10 ng respectively.Recoveries of fenpropathrin and deltamethrin in tea with this analytical method（the concentrations of fenpropathrin and deltamethrin fortified were 0.50～10.0 mg/kg） were 94.8%-108%and 90.5%-102%respectively.The RSDs were 1.71%-6.22%and 3.82%-6.39%, respectively.The precision and accuracy of this method were generally fit for analysis of fenpropathrin and deltamethrin residues in tea.
出处
《中国农学通报》
CSCD
北大核心
2010年第3期238-241,共4页
Chinese Agricultural Science Bulletin
